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0.382 - 0.702
2-oxo-3-methylbutanoate
0.055 - 1.5
2-oxobutanoate
0.125 - 47.69
2-oxopentanoate
10.4 - 40.4
3,3-dimethyl-2-oxobutanoate
27.63
3-methyl-2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
0.385 - 99.38
4-methyl-2-oxopentanoate
37 - 280
alpha-keto-beta-methylvalerate
29 - 280
alpha-keto-isocaproate
16.6
DL-methionine
pH 9, 30°C
14.1
L-arginine
pH 9, 30°C
20.8
L-proline
pH 9, 30°C
33.9
L-tert-leucine
Roseibium aggregatum
-
wild-type, pH 8, 30°C
26.4
L-tyrosine
pH 9, 30°C
13
NADP+
-
mutant enzyme D203A/I204R and D203A/I204R/D210R
0.06 - 0.214
oxo(phenyl)acetate
-
0.006 - 347
trimethylpyruvate
additional information
additional information
-
0.382
2-oxo-3-methylbutanoate
mutant T45M/E116V, pH 9.5, 35°C
0.478
2-oxo-3-methylbutanoate
wild-type, pH 9.5, 35°C
0.659
2-oxo-3-methylbutanoate
mutant E116V, pH 9.5, 35°C
0.702
2-oxo-3-methylbutanoate
mutant T45M, pH 9.5, 35°C
0.055
2-oxobutanoate
-
wild-type, pH 7.5, 37°C
0.056
2-oxobutanoate
-
wild-type, pH 7.5, 37°C
0.149
2-oxobutanoate
wild-type, pH 9.5, 35°C
0.15
2-oxobutanoate
-
mutant Q358N, pH 7.5, 37°C
0.168
2-oxobutanoate
mutant E116V, pH 9.5, 35°C
0.174
2-oxobutanoate
mutant T45M, pH 9.5, 35°C
0.284
2-oxobutanoate
mutant T45M/E116V, pH 9.5, 35°C
0.46
2-oxobutanoate
-
mutant M347G, pH 7.5, 37°C
0.461
2-oxobutanoate
-
mutant M374G, pH 7.5, 37°C
0.826
2-oxobutanoate
-
mutant M347G/Q358N, pH 7.5, 37°C
0.83
2-oxobutanoate
-
mutant M347G/Q358N, pH 7.5, 37°C
1.5
2-oxobutanoate
-
mutant Q358N, pH 7.5, 37°C
24
2-Oxohexanoate
-
mutant enzyme A113A/V291L
110
2-Oxohexanoate
-
wild-type enzyme
140
2-Oxohexanoate
-
mutant enzyme A113A
0.125
2-oxopentanoate
-
pH 8, 50°C
0.129
2-oxopentanoate
mutant T45M/E116V, pH 9.5, 35°C
0.332
2-oxopentanoate
wild-type, pH 9.5, 35°C
0.384
2-oxopentanoate
mutant E116V, pH 9.5, 35°C
0.588
2-oxopentanoate
mutant T45M, pH 9.5, 35°C
47.69
2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
10.4
3,3-dimethyl-2-oxobutanoate
-
pH 7.0, temperature not specified in the publication
19.4
3,3-dimethyl-2-oxobutanoate
-
His-tagged enzyme, pH 9.0, 25°C
31
3,3-dimethyl-2-oxobutanoate
wild-type, pH 10.0, 30°C
34
3,3-dimethyl-2-oxobutanoate
mutant A43V/D124E , pH 10.0, 30°C
40.4
3,3-dimethyl-2-oxobutanoate
-
enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C
0.385
4-methyl-2-oxopentanoate
mutant T45M/E116V, pH 9.5, 35°C
0.467
4-methyl-2-oxopentanoate
wild-type, pH 9.5, 35°C
0.532
4-methyl-2-oxopentanoate
mutant T45M, pH 9.5, 35°C
0.543
4-methyl-2-oxopentanoate
mutant E116V, pH 9.5, 35°C
99.38
4-methyl-2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
37
alpha-keto-beta-methylvalerate
-
mutant enzyme A113A/V291L
235
alpha-keto-beta-methylvalerate
-
mutant enzyme A113A
280
alpha-keto-beta-methylvalerate
-
wild-type enzyme
29
alpha-keto-isocaproate
-
mutant enzyme A113A/V291L
94
alpha-keto-isocaproate
-
mutant enzyme A113A
280
alpha-keto-isocaproate
-
wild-type enzyme
1.4
L-Ile
-
mutant enzyme A113A/V291L
2 - 8
L-Ile
-
wild-type enzyme
3.31
L-Ile
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
23
L-Ile
-
mutant enzyme A113A
0.74
L-Leu
-
mutant enzyme A113A/V291L
2.8
L-Leu
-
mutant enzyme A113A
10.83
L-Leu
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
50
L-Leu
-
wild-type enzyme
2.3
L-leucine
-
pH 9.5, 30°C
4.3
L-leucine
pH 8.5, 30°C
10
L-leucine
-
pH 10.5, 20°C
30.13
L-leucine
pH 9, 30°C
0.18
L-norleucine
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
0.6
L-norleucine
-
mutant enzyme A113A/V291L
1.3
L-norleucine
-
wild-type enzyme
18
L-norleucine
-
mutant enzyme A113A
0.29
L-norvaline
-
mutant enzyme A113A/V291L
1.53
L-norvaline
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
7.7
L-norvaline
-
mutant enzyme A113A
13
L-norvaline
-
wild-type enzyme
0.8
L-Phe
-
mutant enzyme A113A
1.4
L-Phe
-
mutant enzyme A113A/V291L
1.8
NAD+
-
pH 9.5, 30°C
90
NAD+
-
wild-type enzyme
0.286
NADH
mutant T45M/E116V, pH 9.5, 35°C
0.544
NADH
wild-type, pH 9.5, 35°C
0.552
NADH
mutant T45M, pH 9.5, 35°C
1.1
NADH
-
pH 7.0, temperature not specified in the publication
1.3
NADH
mutant E116V, pH 9.5, 35°C
3.1
NADH
-
enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C
5.4
NADH
-
His-tagged enzyme, pH 9.0, 25°C
33.7
NADH
wild-type, pH 8.5, 30°C
35.1
NADH
mutant E24V/E116V, pH 8.5, 30°C
42.6
NADH
mutant E24A/D126E, pH 8.5, 30°C
50.9
NADH
mutant D126E, pH 8.5, 30°C
60.5
NADH
mutant E116V, pH 8.5, 30°C
96
NADH
wild-type, pH 10.0, 30°C
110
NADH
mutant A43V/D124E , pH 10.0, 30°C
347
NADH
Roseibium aggregatum
-
wild-type, pH 8, 30°C
571
NADH
Roseibium aggregatum
-
mutant D153N, pH 8, 30°C
571
NADH
Roseibium aggregatum
-
mutant H191N, pH 8, 30°C
617
NADH
Roseibium aggregatum
-
mutant P130V, pH 8, 30°C
688
NADH
Roseibium aggregatum
-
mutant D153N/H191N, pH 8, 30°C
0.06
oxo(phenyl)acetate
mutant T45M, pH 9.5, 35°C
-
0.101
oxo(phenyl)acetate
mutant E116V, pH 9.5, 35°C
-
0.182
oxo(phenyl)acetate
wild-type, pH 9.5, 35°C
-
0.214
oxo(phenyl)acetate
mutant T45M/E116V, pH 9.5, 35°C
-
9.9
phenylpyruvate
-
mutant enzyme A113A/V291L
22
phenylpyruvate
-
mutant enzyme A113A
0.006
trimethylpyruvate
mutant T45M, pH 9.5, 35°C
0.016
trimethylpyruvate
wild-type, pH 9.5, 35°C
0.033
trimethylpyruvate
mutant E116V, pH 9.5, 35°C
0.036
trimethylpyruvate
mutant T45M/E116V, pH 9.5, 35°C
39.1
trimethylpyruvate
-
pH 9.5, 30°C
45.4
trimethylpyruvate
pH 8.5, 30°C
60.5
trimethylpyruvate
mutant E116V, pH 8.5, 30°C
66.2
trimethylpyruvate
mutant E24V/E116V, pH 8.5, 30°C
77.4
trimethylpyruvate
mutant D126E, pH 8.5, 30°C
93.3
trimethylpyruvate
mutant E24A/D126E, pH 8.5, 30°C
94.1
trimethylpyruvate
wild-type, pH 8.5, 30°C
347
trimethylpyruvate
Roseibium aggregatum
-
wild-type, pH 8, 30°C
additional information
additional information
-
turnover numbers of wild-type enzyme and mutant enzymes
-
additional information
additional information
-
turnover numbers of wild-type enzyme and mutant enzymes
-
additional information
additional information
-
turnover numbers of wild-type enzyme and mutant enzymes
-
1.4.1.9 leucine dehydrogenase
results (
results (
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