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1.4.3.10: putrescine oxidase

This is an abbreviated version!
For detailed information about putrescine oxidase, go to the full flat file.

Word Map on EC 1.4.3.10

Reaction

putrescine
+
O2
+
H2O
=
4-aminobutanal
+
NH3
+
H2O2

Synonyms

adenine dinucleotide-containing putrescine oxidase, APUO, oxidase, putrescine, PO, PuO, PUOX, PUT oxidase, PutOx, putrescine oxidase, Re-PuO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.10 putrescine oxidase

Engineering

Engineering on EC 1.4.3.10 - putrescine oxidase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A394C
A394C/A396T/Q431G
mutant with strongly reduced catalytic efficiency
A394C/V433M
the mutations improve the affinity toward FAD
E324A
the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme
E324L
the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme
P15I/A394C
the mutations improve the affinity toward FAD
A394C
-
active mutant with covalently bound FAD
-
E324A
-
the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme
-
E324L
-
the mutant enzyme shows activity with monoamines, which are not accepted by the wild type enzyme
-