1.4.3.14: L-lysine oxidase
This is an abbreviated version!
For detailed information about L-lysine oxidase, go to the full flat file.
Word Map on EC 1.4.3.14
Reaction
Synonyms
AIP, apoptosis-inducing protein, L-LOX/MOG, L-lysine alpha-oxidase, L-lysine oxidase/monooxygenase, L-lysine-a-oxidase, L-lysine-alpha-oxidase, L-lysyl-alpha-oxidase, L6150, LyOx, lysine-oxidase, LysOx
ECTree
1.4.3.14 L-lysine oxidaseAdvanced search results
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Engineering on EC 1.4.3.14 - L-lysine oxidase
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
D212A
the Km-value of the mutant enzyme for L-lysine is increased 43fold respectively, to the wild-type enzyme, the kcat-value is slightly decreased. The single mutation does not much affect substrate preference
D212A/D315A
the Km value of the double mutant variant increases 585fold and kcat/Km decreasea more than 2000fold to the wild-type enzyme. The D212A/D315A double mutation increases relative activity for aromatic L-amino acids (L-phenylalanine, L-tyrosine, and L-tryptophan) and L-arginine while reduced for L-lysine
D315A
the Km-value of the mutant enzyme for L-lysine is increased 22fold respectively, to the wild-type enzyme, the kcat-value is slightly decreased. The single mutation does not much affect substrate preference
M67A
activity is greatly reduced and is too low to determine the kinetic parameters for L-lysine
