1.4.3.19: glycine oxidase
This is an abbreviated version!
For detailed information about glycine oxidase, go to the full flat file.
Word Map on EC 1.4.3.19
-
1.4.3.19
-
d-amino
-
sarcosine
-
flavoproteins
-
glyphosate
-
flavin
-
fad
-
tryptophylquinone
-
flavoenzyme
-
herbicide
-
d-proline
-
homotetrameric
-
d-alanine
-
pseudoalteromonas
-
luteoviolacea
-
protein-derived
-
thios
-
fad-dependent
-
site-saturation
-
fad-binding
-
quinoprotein
-
ttq
-
half-reaction
-
5-enolpyruvylshikimate-3-phosphate
-
fad-containing
- 1.4.3.19
-
d-amino
- sarcosine
- flavoproteins
- glyphosate
- flavin
- fad
-
tryptophylquinone
-
flavoenzyme
-
herbicide
- d-proline
-
homotetrameric
- d-alanine
-
pseudoalteromonas
- luteoviolacea
-
protein-derived
-
thios
-
fad-dependent
-
site-saturation
-
fad-binding
-
quinoprotein
- ttq
-
half-reaction
- 5-enolpyruvylshikimate-3-phosphate
-
fad-containing
Reaction
Synonyms
BcGO, glycin oxidase, glycine:oxygen oxidoreductase (deaminating), GlyOX, GO, GOX, GoxA, GOXK, GoxR, LodA, PlGoxA, ThiO
ECTree
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KM Value
KM Value on EC 1.4.3.19 - glycine oxidase
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4 - 10
-
apparent value for mutant enzyme M261Y, at 25°C and pH 8.5
20.6
D-alanine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
34.65
D-alanine
wild type enzyme, at pH 7.5, temperature not specified in the publication
217
D-alanine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
315
D-alanine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
2000
D-alanine
-
Km above 2000 mM, apparent value for mutant enzyme M261H, at 25°C and pH 8.5
221.8
D-pipecolate
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
290
D-pipecolate
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
2000
D-pipecolate
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
7.9
D-proline
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
8.9
D-proline
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
9.3
D-proline
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.25
glycine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.4
glycine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.53
glycine
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
0.6
glycine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.79
glycine
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
0.826
glycine
-
mutant enzyme F237A, at 30°C, pH not specified in the publication
0.95
glycine
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
1.04
glycine
wild type enzyme, at pH 7.5, temperature not specified in the publication
1.41
glycine
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
2.25
glycine
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
3.03
glycine
-
mutant enzyme F237Y, at 30°C, pH not specified in the publication
39
glycine
-
wild type enzyme, at 30°C, pH not specified in the publication
41.55
glycine
mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C
41.64
glycine
mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C
80.43
glycine
mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C
101.8
glycine
mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C
134.4
glycine
mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C
138.1
glycine
mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C
0.52
glycine-ethyl-ester
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.6
glycine-ethyl-ester
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
1.8
glycine-ethyl-ester
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.53
glyphosate
mutant enzyme G51R/G60S/T118A/K133R/I198V/V262I/I284L/L307S/E357G, at pH 8.5 and 60°C
0.57
glyphosate
mutant enzyme G51R/D60G/T118A/K133R/I284L, at pH 8.5 and 60°C
1.37
glyphosate
mutant enzyme G51R/G60S/D121G/S122P/H164Y/M267T, at pH 8.5 and 60°C
1.69
glyphosate
mutant enzyme G51R/G60S/K133R/S210P/R250G/V262I, at pH 8.5 and 60°C
2.17
glyphosate
mutant enzyme G51R/D60G/T118A/K133R, at pH 8.5 and 60°C
2.77
glyphosate
mutant enzyme G51R/D60G/K133R/V262I, at pH 8.5 and 60°C
3.8
glyphosate
mutant enzyme BG51R/G60S/I198V/E357G, at pH 8.5 and 60°C
4.37
glyphosate
mutant enzyme BG51R/G60S/S210P/M267T, at pH 8.5 and 60°C
10.44
glyphosate
mutant enzyme N336R, at pH 7.5, temperature not specified in the publication
22.45
glyphosate
mutant enzyme N336K, at pH 7.5, temperature not specified in the publication
42.31
glyphosate
mutant enzyme N336H, at pH 7.5, temperature not specified in the publication
68.36
glyphosate
mutant enzyme N336A, at pH 7.5, temperature not specified in the publication
84.79
glyphosate
wild type enzyme, at pH 7.5, temperature not specified in the publication
96.73
glyphosate
mutant enzyme N336G, at pH 7.5, temperature not specified in the publication
0.22
N-ethylglycine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.5
N-ethylglycine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
2.8
N-ethylglycine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.416
O2
-
mutant enzyme F237A, at 30°C, pH not specified in the publication
2.27
O2
-
mutant enzyme F237Y, at 30°C, pH not specified in the publication
2.6
O2
-
wild type enzyme, at 30°C, pH not specified in the publication
0.45
sarcosine
-
in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate, at pH 8.5 and 37°C
0.51
sarcosine
mutant enzyme I15V, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
0.6
sarcosine
wild type enzyme, in 0.4 mM 4-aminoantipyrine, 7 mM phenol, 4.4 U/ml horseradish peroxidase, 0.0002 mM FAD, and 75 mM sodium diphosphate (pH 8.5) at 37°C
1.51
sarcosine
wild type enzyme, at pH 7.5, temperature not specified in the publication