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1.4.3.19: glycine oxidase

This is an abbreviated version!
For detailed information about glycine oxidase, go to the full flat file.

Word Map on EC 1.4.3.19

Reaction

2-iminoacetate
+
H2O
=
glyoxylate
+
NH3

Synonyms

BcGO, glycin oxidase, glycine:oxygen oxidoreductase (deaminating), GlyOX, GO, GOX, GoxA, GOXK, GoxR, LodA, PlGoxA, ThiO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.19 glycine oxidase

Specific Activity

Specific Activity on EC 1.4.3.19 - glycine oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.19
-
crude extract
0.21
-
purification step ultrafiltration
0.568
-
wild-type enzyme
0.9
mutant enzyme I15V, from crude extract
1.06
-
His-tagged chimeric glycine oxidase
1.2
-
purification step HiTrap chelating chromatography
1.75
-
crude extract, at 37°C and pH 8.5
1000
after 68.6fold purification, when D-alanine is used as a substrate, at pH 8.0 and 37°C
11.85
14
cell free extract, when glycine is used as a substrate, at pH 8.0 and 37°C
8.6
mutant enzyme I15V, after 9.3fold purification
960
after 68.6fold purification, when glycine is used as a substrate, at pH 8.0 and 37°C