1.4.3.2: L-amino-acid oxidase

This is an abbreviated version, for detailed information about L-amino-acid oxidase, go to the full flat file.

Reaction

an L-amino acid
+
H2O
+
O2
=
a 2-oxo carboxylate
+
NH3
+
H2O2

Synonyms

AAD, ACTX-6, ACTX-8, Akbu-LAAO, APIT, aromatic L-amino acid oxidase, Balt-LAAO-I, Bpir-LAOO-1, DAA, Dolabellanin, escapin, IL4I1, Il4i1 protein, ink toxin 1, Interleukin Four Induced Gene 1 protein, L-AAO, L-AAO/MOG, L-AAO1, L-amino acid oxidase, L-amino acid: O2 oxidoreductase, L-amino acid:O2 oxidoreductase, L-amino acid:O2 oxidoreductase (deaminating), L-aminooxidase, L-Aox, L-phenylalanine oxidase, LAAO, LAAO-I, LAAO-II, LAAO1, LAAOI, LAAOII, LAO, M-LAO, ophio-amino-acid oxidase, Pm1, RoLAAO, sarA, Sebastes schlegeli antibacterial protein, Sebastes schlegelii antibacterial protein, SSAP, TJ-LAO, toxophallin, TSV-LAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase

Cloned

Cloned on EC 1.4.3.2 - L-amino-acid oxidase

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Cloned/COMMENTARY
ORGANISM
UNIPROT
LITERATURE
; into the pT7Blue T-vector
DNA and amino acid sequence determination and analysis, expression of His-tagged enzyme in Escherichia coli strain Rosetta 2 (lambdaDE3)
-
DNA and amino acid sequence determination and analysis, phylogenetic analysis
expressed in Escherichia coli BL21 CodonPlus (DE)-RP and Arctic Express (DE) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli JM109 cells and in Pichia pastoris strain KM71H
-
expressed in Pichia pastoris strain GS115
-
expression in Escherichia coli
-
expression in Streptomyces lividans; expression into different Escherchia coli and Streptomyces lividans vectors. Expression in Escherichia coli results in an insoluble protein whereas Steptomyces lividans is a suitable host for the heterologous production of L-AAO
into pGEM-T vector
isozymes MPLAO1, MPLAO2, and MPLAO3, DNA and amino acid sequence determinhation and analysis, phylogenetic analysis and sequence comparisons
LAAO open reading frame inserted after the alpha-MF-signal sequence. Expressed in Pichia pastoris
-
overexpressed in Escherichia coli BL21(DE3)pLysS
-
xcpression in Escherichia coli
-