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1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO,  LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

Purification

Purification on EC 1.4.3.3 - D-amino-acid oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography on Cibacron Blue Sepharose
-
ammonium sulfate precipitation, Toyopearl butyl column chromatography, and Q Sepharose column chromatography
-
enzyme intracellularly expressed under P(GAP) integrant in Pichia pastoris
-
His-Bind column chromatography
Hitrap chelating chromatography
HiTrap DEAE column chromatography
-
large scale purification of apo-D-aminoacid oxidase
-
Mono Q HR 10/10 column chromatography and Sephacryl S-200 gel filtration
-
MonoQ column chromatography
-
MonoQ column chromatography and Sephadex G-25 gel filtration
-
mutant enzyme M213R
Ni-NTA column chromatography
Ni-NTA column chromatography and gel filtration
Ni2+-chelate affinity column chromatography
-
partial
partial purification by heat treatment at 53°C
phenyl-Sepharose column chromatography
Q-Sepharose column chromatography and hydroxyapatite column chromatography
recombinant DAAO from Spodoptera frugiperda Sf9 cells
recombinant DAO from Escherichia coli to homogeneity
-
recombinant DAo from Pichia pastoris strain CBS 7435, the yeast cells are permeabilized by freeze-drying and incubation in 10% v/v 2-propanol resulting in a highly active, 1.6 kUnits/g dry matter, and stable oxidase preparation
recombinant enzyme
recombinant enzyme by ammonium sulfate fractionation, anion exchange chromatography, and dialysis
-
recombinant enzyme from Escherichia coli
-
recombinant enzyme from Escherichia coli strain BL21(DE3)
-
recombinant enzyme fused to the maltose-binding protein from Escherichia coli strain TB1 by maltose affinity chromatography
recombinant N-terminally Strep-tagged enzyme to homogeneity from Escherichia coli strain BL21(DE3) by affinity chromatography
-
recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) by ultracentrifugation, affinity and anion exchange chromatography
-
recombinant wild-type enzyme and mutants from Escherichia coli BL21(DE3)
Resource-Q anion exchange column chromatography
Talon metal affinity column chromatography, and Superdex 200 gel filtration
UNO Q6 column chromatography, and Superdex 200 gel filtration
-
use of surface layer (S-layer) protein of Lactobacillus brevis as a self-aggregating protein tag to enable cost-effective separation of human and yeast D-amino acid oxidases expressed in Escherichia coli cells. Human and yeast D-amino acid oxidases fused with S-layer proteins can be easily separated by aggregates at the interface and/or in a few conditions by precipitates to the bottom of the PEG-phosphate aqueous system
wild-type enzyme and DELTAloop mutant
-