1.4.4.2: glycine dehydrogenase (aminomethyl-transferring)

This is an abbreviated version!
For detailed information about glycine dehydrogenase (aminomethyl-transferring), go to the full flat file.

Word Map on EC 1.4.4.2

Reaction

glycine
+
[glycine-cleavage complex H protein]-N6-lipoyl-L-lysine
=
[glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine
+
CO2

Synonyms

decarboxylase, glycine, GDC, GDCP, GLDC, GLDH, GLDP, Gly decarboxylase complex, Gly decarboxylase H1, glycine cleavage enzyme complex, glycine cleavage H protein, glycine cleavage system H protein 1, Glycine cleavage system P-protein, glycine decarboxylase, glycine decarboxylase (P-protein), glycine decarboxylase complex, glycine decarboxylase complex H, glycine decarboxylase P-protein, glycine dehydrogenase, glycine dehydrogenase (decarboxylating), glycine-cleavage complex, H protein, H-protein, H1 protein, H2 protein, L protein, More, P protein, P-protein, P-protein (glycine decarboxylase), P-subunit, Protein P1, T protein

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.4 With a disulfide as acceptor
                1.4.4.2 glycine dehydrogenase (aminomethyl-transferring)

Purification

Purification on EC 1.4.4.2 - glycine dehydrogenase (aminomethyl-transferring)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
H1, H2, and L protein purified to homogeneity, more than 95% pure; using a nickel-nitrilotriacetic acid-agarose column
-
Ni-NTA column chromatography
Q-Sepharose column chromatography and Sephacryl S-100 gel filtration
-
recombinant His-tagged enzyme from Escherichia coli by nickel affinity and ion exchange chromatography, and gel filtration
recombinant His6-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
-
ultracentrifugation
-