1.4.5.1: D-amino acid dehydrogenase (quinone)
This is an abbreviated version!
For detailed information about D-amino acid dehydrogenase (quinone), go to the full flat file.
Reaction
Synonyms
DadA
ECTree
1.4.5.1 D-amino acid dehydrogenase (quinone)Advanced search results
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results (Substrates Products
Substrates Products on EC 1.4.5.1 - D-amino acid dehydrogenase (quinone)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
beta-chloro-D-alanine + oxidized acceptor + H2O
? + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 20% of the activity with D-alanine
-
-
?
D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol
D-Ala + oxidized coenzyme Q1 + H2O
pyruvate + NH3 + reduced coenzyme Q1
specific activity is 37% of that with D-proline
-
-
?
D-alanine + oxidized 2,6-dichloroindophenol + H2O
pyruvate + NH3 + reduced 2,6-dichloroindophenol
D-alanine + oxidized 2,6-dichlorophenolindophenol + H2O
pyruvate + NH3 + reduced 2,6-dichlorophenolindophenol
-
r
-
-
?
D-alanine + oxidized acceptor
pyruvate + NH3 + reduced acceptor
-
the enzyme couples D-alanine oxidation to solute active transport in Escherichia coli B cytoplasmic membrane vesicles
-
-
?
D-alanine + oxidized coenzyme Q1
pyruvate + NH3 + reduced coenzyme Q1
-
no activity with menaquinone or adriamycin
-
-
?
D-arginine + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxo-5-guanidinopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
41% of the activity with D-alanine
-
-
?
D-arginine + oxidized acceptor + H2O
2-oxo-5-guanidinopentanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 2.9% of the activity with D-alanine
-
-
?
D-asparagine + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
D-asparagine + oxidized acceptor + H2O
2-oxosuccinamic acid + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 21% of the activity with D-alanine
-
-
?
D-cycloserine + oxidized 2,6-dichloroindophenol + H2O
?
-
as a active as D-alanine
-
-
?
D-cysteine + oxidized acceptor
? + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 4.1% of the activity with D-alanine
-
-
?
D-ethionine + oxidized acceptor + H2O
? + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 41% of the activity with D-alanine
-
-
?
D-glutamine + oxidized 2,6-dichloroindophenol + H2O
4-carbamoyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
D-histidine + oxidized 2,6-dichloroindophenol + H2O
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
-
8.5% of the activity compared to D-alanine
-
-
?
D-histidine + oxidized acceptor + H2O
3-(1H-imidazol-4-yl)-2-oxopropanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 12% of the activity with D-alanine
-
-
?
D-leucine + oxidized acceptor + H2O
4-methyl-2-oxopentanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 20% of the activity with D-alanine
-
-
?
D-methionine + oxidized 2,6-dichloroindophenol + H2O
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
D-methionine + oxidized 2,6-dichlorophenolindophenol + H2O
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
70% of the activity with D-alanine
-
-
?
D-methionine + oxidized acceptor + H2O
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 39% of the activity with D-alanine
-
-
?
D-Phe + oxidized coenzyme Q1 + H2O
phenylpyruvate + NH3 + reduced coenzyme Q1
specific activity is 20% of that with D-proline
-
-
?
D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O
phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol
D-phenylalanine + oxidized acceptor + H2O
phenylpyruvate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 72% of the activity with D-alanine
-
-
?
D-proline + oxidized 2,6-dichloroindophenol
DELTA1-pyrroline-2-carboxylate + reduced 2,6-dichloroindophenol
-
28% of the activity compared to D-alanine
-
-
?
D-proline + oxidized 2,6-dichlorophenolindophenol
DELTA1-pyrroline-2-carboxylate + NH3 + reduced 2,6-dichlorophenolindophenol
-
19% of the activity with D-alanine
-
-
?
D-proline + oxidized acceptor
DELTA1-pyrroline-2-carboxylate + reduced acceptor
-
assayed in toluenized cells, reaction rate is 2.6% of the activity with D-alanine
-
-
?
D-proline + oxidized coenzyme Q1 + H2O
DELTA1-pyrroline-2-carboxylate + NH3 + reduced coenzyme Q1
-
-
-
?
D-Ser + oxidized coenzyme Q1 + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced coenzyme Q1
specific activity is 7% of that with D-proline
-
-
?
D-serine + oxidized 2,6-dichloroindophenol + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichloroindophenol
-
29% of the activity compared to D-alanine
-
-
?
D-serine + oxidized 2,6-dichlorophenolindophenol + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
20% of the activity compared to D-alanine
-
-
?
D-serine + oxidized acceptor + H2O
3-hydroxy-2-oxopropanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 13% of the activity with D-alanine
-
-
?
D-threonine + H2O + oxidized acceptor
3-hydroxy-2-oxobutanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 5.9% of the activity with D-alanine
-
-
?
D-valine + oxidized 2,6-dichloroindophenol + H2O
2-oxoisopentanoate + NH3 + reduced 2,6-dichloroindophenol
-
11% of the activity compared to D-alanine
-
-
?
D-valine + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxoisopentanoate + NH3 + reduced 2,6-dichlorophenolindophenol
-
26% of the activity with D-alanine
-
-
?
D-valine + oxidized acceptor + H2O
2-oxoisopentanoate + NH3 + reduced acceptor
-
assayed in toluenized cells, reaction rate is 2.6% of the activity with D-alanine
-
-
?
additional information
?
-
activity with D-Val, D-Met, D-Glu, D-Asp, L-Pro and L-Ala is below 5% of the activity with D-Pro
-
-
?
3,4-dehydro-DL-proline + oxidized 2,6-dichloroindophenol
?
-
when membrane fractions from Escherichia coli strain UMM5 (putA1::Tn5 proC24) lacking both L-proline dehydrogenase and L-DELTA1-pyrroline-5-carboxylate reductase are incubated with 3,4-dehydro-DL-proline, pyrrole-2-carboxylate is formed. Oxidation of 3,4-dehydro-DL-proline by membrane fractions from strain UMM5 is induced by growth in minimal medium containing D- or L-alanine. An Escherichia coli strain with no D-alanine dehydrogenase activity due to the dadA237 mutation is unable to oxidize either 3,4-dehydro-D-proline or D-alanine, as are spontaneous Dad- mutants of Escherichia coli strain UMM5. Membrane fractions containing D-alanine dehydrogenase also catalyze the oxidation of D-2-aminobutyrate, D-norvaline, D-norleucine, cis-4-hydroxy-D-proline, and DL-ethionine. D-Alanine dehydrogenase is responsible for the residual 3,4-dehydro-DL-proline oxidation activity in putA proC mutants of Escherichia coli. This enzyme plays a general role in the metabolism of D-amino acids and their analogues
-
-
?
3,4-dehydro-DL-proline + oxidized 2,6-dichloroindophenol
?
-
when membrane fractions from Escherichia coli strain UMM5 (putA1::Tn5 proC24) lacking both L-proline dehydrogenase and L-DELTA1-pyrroline-5-carboxylate reductase are incubated with 3,4-dehydro-DL-proline, pyrrole-2-carboxylate is formed. Oxidation of 3,4-dehydro-DL-proline by membrane fractions from strain UMM5 is induced by growth in minimal medium containing D- or L-alanine. An Escherichia coli strain with no D-alanine dehydrogenase activity due to the dadA237 mutation is unable to oxidize either 3,4-dehydro-D-proline or D-alanine, as are spontaneous Dad- mutants of Escherichia coli strain UMM5. Membrane fractions containing D-alanine dehydrogenase also catalyze the oxidation of D-2-aminobutyrate, D-norvaline, D-norleucine, cis-4-hydroxy-D-proline, and DL-ethionine. D-Alanine dehydrogenase is responsible for the residual 3,4-dehydro-DL-proline oxidation activity in putA proC mutants of Escherichia coli. This enzyme plays a general role in the metabolism of D-amino acids and their analogues
-
-
?
D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol
-
38% of the activity compared to D-alanine
-
-
?
D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol
-
89% of the activity compared to D-alanine
-
-
?
D-2-amino-n-butyrate + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxobutyrate + NH3 + reduced 2,6-dichloroindophenol
-
81% of the activity with D-alanine
-
-
?
D-alanine + oxidized 2,6-dichloroindophenol + H2O
pyruvate + NH3 + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-alanine + oxidized 2,6-dichloroindophenol + H2O
pyruvate + NH3 + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-alanine + oxidized 2,6-dichloroindophenol + H2O
pyruvate + NH3 + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-alanine + oxidized acceptor + H2O
pyruvate + NH3 + reduced acceptor
-
the enzyme is inducible by L-alanine and repressible by glucose
-
-
?
D-alanine + oxidized acceptor + H2O
pyruvate + NH3 + reduced acceptor
-
assayed in toluenized cells, formation of pyruvate is determined using 2,4-dinitrophenylhydrazine
-
-
?
D-asparagine + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
26% of the activity compared to D-alanine
-
-
?
D-asparagine + oxidized 2,6-dichlorophenolindophenol + H2O
2-oxosuccinamic acid + NH3 + reduced 2,6-dichlorophenolindophenol
-
96% of the activity with D-alanine
-
-
?
D-glutamine + oxidized 2,6-dichloroindophenol + H2O
4-carbamoyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
-
-
-
-
?
D-glutamine + oxidized 2,6-dichloroindophenol + H2O
4-carbamoyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
-
52% of the activity with D-alanine
-
-
?
D-methionine + oxidized 2,6-dichloroindophenol + H2O
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
-
35% of the activity compared to D-alanine
-
-
?
D-methionine + oxidized 2,6-dichloroindophenol + H2O
4-methylsulfanyl-2-oxobutanoate + NH3 + reduced 2,6-dichloroindophenol
-
97% of the activity compared to D-alanine
-
-
?
D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O
phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol
-
as active as D-alanine
-
-
?
D-phenylalanine + oxidized 2,6-dichloroindophenol + H2O
phenylpyruvate + NH3 + reduced 2,6-dichloroindophenol
-
63% of the activity with D-alanine
-
-
?
