1.5.1.11: D-octopine dehydrogenase
This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.
Reaction
Synonyms
D-Octopine synthase, Dehydrogenase, octopine, flavin-containing opine dehydrogenase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, OcDH, OCS, Octopine dehydrogenase, Octopine synthase, Octopine:NAD oxidoreductase, ODH, pTi_008
ECTree
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Purification
Purification on EC 1.5.1.11 - D-octopine dehydrogenase
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ammonium sulfate precipitation, Ni2+-NTA column chromatography, and Sephadex G-100 gel filtration
crown-gall tumor-tissue induced by Agrobacterium tumefaciens
His5-tagged OcDH and His6-tagged OcDH are purified by Ni-NTA chromatography, yielding almost 20 mg homogenous enzyme per litre of cell culture (purity greater than 98%). The purification of OcDH-tagless requires several chromatographic steps and yields 3-5 mg per litre of cell culture, with a purity of more than 98%
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, ultrafiltration, dialysis, and gel filtration
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the biospecific chromatographic affinity purification is not applicable to the purification of the enzyme from Monodonta lineate
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the biospecific chromatographic affinity purification is not applicable to the purification of the enzyme from Mytilus edulis
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