1.5.1.11: D-octopine dehydrogenase
This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.
Word Map on EC 1.5.1.11
Reaction
Synonyms
D-Octopine synthase, Dehydrogenase, octopine, flavin-containing opine dehydrogenase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, OcDH, OCS, Octopine dehydrogenase, Octopine synthase, Octopine:NAD oxidoreductase, ODH, pTi_008
ECTree
1.5.1.11 D-octopine dehydrogenaseAdvanced search results
results (
results (Substrates Products
Substrates Products on EC 1.5.1.11 - D-octopine dehydrogenase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
canavanine + pyruvate + NADH
? + NAD+ + H2O
24.74% activity with canavanine compared to L-Arg
-
-
?
Canavanine + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-canavanine + NADP+ + H2O
-
at 56% of the activity with L-Arg
-
-
?
Homoarginine + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-homoarginine + NADP+ + H2O
-
at 62% of the activity with L-Arg
-
-
?
L-alanine + pyruvate + NADH
? + NAD+ + H2O
0.29% activity with L-alanine compared to L-Arg
-
-
?
L-Arg + 2-oxobutanoate + NADPH
N2-(D-2-Carboxypropyl)-L-Arg + NADP+ + H2O
-
at 21% of the activity with pyruvate
-
-
?
L-Arg + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
100% activity with L-Arg
-
-
?
L-canavanine + pyruvate + NADPH + H+
N2-(D-1-carboxyethyl)-canavanine + NADP+ + H2O
-
-
-
-
r
L-Citrulline + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-citrulline + NADP+ + H2O
-
-
-
-
?
L-Cys + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Cys + NADP+ + H2O
-
at 27% of the activity with L-Arg
-
-
?
L-cysteine + pyruvate + NADH
? + NAD+ + H2O
1.18% activity with L-cysteine compared to L-Arg
-
-
?
L-His + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-His + NADP+ + H2O
-
at 78% of the activity with L-Arg
-
-
?
L-serine + pyruvate + NADPH
N2-(D-1-carboxyethyl)-L-Ser + NADP+ + H2O
-
at 25% of the activity with L-Arg
-
-
?
norvaline + pyruvate + NADH
? + NAD+ + H2O
0.15% activity with norvaline compared to L-Arg
-
-
?
Orn + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Orn + NADP+ + H2O
-
at 30% of the activity with L-Arg
-
-
?
ornithine + pyruvate + NADH
? + NAD+ + H2O
0.26% activity with ornithine compared to L-Arg
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
-
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
-
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
-
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
L-canavanine, at 56% of the activity with L-Arg
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
-
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
57.6-92.6% of the activity with Arg, depending on enzyme variant
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
-
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
L-canavanine, at 48% of the activity with L-Arg
-
-
?
Canavanine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-canavanine + NAD+ + H2O
-
-
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
-
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
-
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
-
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
at 13% of the activity with L-Arg
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
-
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
58.6-70.8% of activity with Arg, depending on enzyme variant
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
-
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
at 22% of the activity with L-Arg
-
-
?
Homoarginine + pyruvate + NADH
N2-(D-1-Carboxyethyl)-homoarginine + NAD+ + H2O
-
-
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
-
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
-
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
-
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
at 41% of the activity with L-Arg
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
-
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
20.6-29.0% of activity with Arg
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
-
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
at 28% of the activity with pyruvate
-
-
?
L-Arg + 2-oxobutanoate + NADH
N2-(D-2-Carboxypropyl)-L-Arg + NAD+ + H2O
-
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
r
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
r
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
2 enzyme forms. One catalyzes the reverse reaction with NAD+, the second with NADP+
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
r
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
Chlamys opercularis
-
-
i.e. octopine
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
r
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADH
N2-(D-1-carboxyethyl)-L-Arg + NAD+ + H2O
-
-
-
?
L-Arg + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O
-
-
-
-
?
L-Arg + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O
-
2 enzyme forms. One catalyzes the reverse reaction with NAD+, the second with NADP+
-
-
?
L-Arg + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Arg + NADP+ + H2O
-
-
-
-
?
L-arginine + pyruvate + NADH + H+
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
-
-
-
?
L-arginine + pyruvate + NADH + H+
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
the reduction reaction is highly preferred
-
-
?
L-arginine + pyruvate + NADH + H+
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
-
-
-
?
L-arginine + pyruvate + NADH + H+
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
highest affinity for L-arginine
-
-
?
L-arginine + pyruvate + NADH + H+
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
-
-
-
r
L-arginine + pyruvate + NADH + H+
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
-
-
-
-
?
L-Gln + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O
-
at 18% of the activity with L-Arg
-
-
?
L-Gln + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Gln + NADP+ + H2O
-
-
-
-
?
L-Lys + pyruvate + NADH
Lysopine + NAD+ + H2O
-
at 1% of the activity with L-Arg
-
-
?
L-Lys + pyruvate + NADH
Lysopine + NAD+ + H2O
-
87.2-92.6% of activity with Arg, depending on enzyme variant
-
-
?
L-Lys + pyruvate + NADH
Lysopine + NAD+ + H2O
-
at 2% of the activity with L-Arg
-
-
?
L-Lys + pyruvate + NADPH
Lysopine + NADP+ + H2O
-
at 31% of the activity with L-Arg
-
-
?
L-Met + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O
-
at 67% of the activity with L-Arg
-
-
?
L-Met + pyruvate + NADPH
N2-(D-1-Carboxyethyl)-L-Met + NADP+ + H2O
-
-
-
-
?
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
L-arginine + pyruvate + NADH + H+
-
-
-
r
N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O
L-arginine + pyruvate + NADH + H+
-
-
-
-
r
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
no activity
-
-
?
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
-
-
-
?
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
at 2% of the activity with L-Arg
-
-
?
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
-
-
-
?
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
at 4% of the activity with L-Arg
-
-
?
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
no activity
-
-
?
Orn + pyruvate + NADH
N2-(D-1-Carboxyethyl)-L-Orn + NAD+ + H2O
-
activity with brain enzyme, no activity with mantle enzyme
-
-
?
additional information
?
-
-
octopine [N2-(1-D-carboxyethyl)-L-arginine] is synthesized by a NAD(P)H-dependent soluble dehydrogenase that catalyzes the reductive condensation of pyruvate with L-arginine. Although the reaction may be reversible in vitro, the frequent use of the term synthase rather than dehydrogenase has emphasized the importance of biosynthesis, but not degradation, and distinguishes it from the mollusk octopine dehydrogenase
-
-
?
additional information
?
-
-
the enzyme OCS utilizes not only L-arginine, but also other amino acids, to yield the corresponding members of the octopine family. Analysis of specificity of electron acceptors using 4-iodonitrotetrazolium violet (INT) or nitroblue tetrazolium (NBT) together with phenazine methosulfate (PMS) (electron-transfer intermediate), ferricyanide, horse heart cytochrome c, and NAD(P)+
-
-
?
additional information
?
-
-
the enzyme may synthesize lysopine, octopinic acid, and the corresponding Nalpha-derivatives of glutamine
-
-
?
additional information
?
-
-
the evolutionary development of the enzyme appears to have led from a broadly specific imino acid dehydrogenase in sea anemones to enzymes increasingly specific for the substrate L-Arg, and pyruvate only. This trend is correlated with an increasing importance of the enzyme in glycolytic redox balance in working muscle and an increased dependence on muscle arginine phosphate reserves for rapid energy generation in higher invertebrate groups
-
-
?
additional information
?
-
-
the evolutionary development of the enzyme appears to have led from a broadly specific imino acid dehydrogenase in sea anemones to enzymes increasingly specific for the substrate L-Arg, and pyruvate only. This trend is correlated with an increasing importance of the enzyme in glycolytic redox balance in working muscle and an increased dependence on muscle arginine phosphate reserves for rapid energy generation in higher invertebrate groups
-
-
?
additional information
?
-
-
octopine is produced in the mantle via the reaction of the muscle isoenzyme and is subsequently flushed out into the blood and transported to other tissues such as the optic lobe for re-oxidation via the optic lobe isoenzyme
-
-
?
additional information
?
-
-
the evolutionary development of the enzyme appears to have led from a broadly specific imino acid dehydrogenase in sea anemones to enzymes increasingly specific for the substrate L-Arg, and pyruvate only. This trend is correlated with an increasing importance of the enzyme in glycolytic redox balance in working muscle and an increased dependence on muscle arginine phosphate reserves for rapid energy generation in higher invertebrate groups
-
-
?
additional information
?
-
-
the enzyme shows no activity when taurine or beta-alanine is used as substrate
-
-
?
additional information
?
-
-
the major role of the brain enzyme may be the oxidation of octopine
-
-
?
additional information
?
-
-
the evolutionary development of the enzyme appears to have led from a broadly specific imino acid dehydrogenase in sea anemones to enzymes increasingly specific for the substrate L-Arg, and pyruvate only. This trend is correlated with an increasing importance of the enzyme in glycolytic redox balance in working muscle and an increased dependence on muscle arginine phosphate reserves for rapid energy generation in higher invertebrate groups
-
-
?
additional information
?
-
-
mantle muscle enzyme appears geared for the rapid synthesis of octopine under conditions of muscular work
-
-
?
additional information
?
-
-
major physiological role in glycolytic energy poduction during burst swimming
-
-
?
