1.5.1.2: pyrroline-5-carboxylate reductase
This is an abbreviated version!
For detailed information about pyrroline-5-carboxylate reductase, go to the full flat file.
Reaction
Synonyms
1-pyrroline-5-carboxylate reductase, aldimine reductase, DELTA1-pyrroline-5-carboxylate reductase, DELTA1-pyrroline-5-carboxylate reductase 2, L-proline oxidase, L-proline-NAD(P)+ 5-oxidoreductase, L-proline:NAD(P)+ 5-oxidoreductase, MTR_7g090160, NADPH-L-DELTA'-pyrroline carboxylic acid reductase, P5C reductase, P5CDH, P5CR, PO, POX, PROC, proline oxidase, Pycr1, PYCR2, Pyr5C reductase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate reductase, reductase, pyrroline-5-carboxylate, TcCLB.509207.90
ECTree
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Purification
Purification on EC 1.5.1.2 - pyrroline-5-carboxylate reductase
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ammonium sulfate precipitation, anion exchange column chromatography, and gel filtration
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by Ni-NTA His-binding resin affinity chromatography, the N-terminal fusion partner is cleaved by enterokinase
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copurification with proline oxidase, both activities located on the same protein
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homogeneity
partial
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography, dialysis, and ultrafiltration
using a HiTrap Ni-NTA column