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1.5.1.2: pyrroline-5-carboxylate reductase

This is an abbreviated version!
For detailed information about pyrroline-5-carboxylate reductase, go to the full flat file.

Word Map on EC 1.5.1.2

Reaction

L-proline
+
NAD(P)+
=
1-pyrroline-5-carboxylate
+
NAD(P)H
+
H+

Synonyms

1-pyrroline-5-carboxylate reductase, aldimine reductase, DELTA1-pyrroline-5-carboxylate reductase, DELTA1-pyrroline-5-carboxylate reductase 2, L-proline oxidase, L-proline-NAD(P)+ 5-oxidoreductase, L-proline:NAD(P)+ 5-oxidoreductase, MTR_7g090160, NADPH-L-DELTA'-pyrroline carboxylic acid reductase, P5C reductase, P5CDH, P5CR, PO, POX, PROC, proline oxidase, Pycr1, PYCR2, Pyr5C reductase, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate reductase, reductase, pyrroline-5-carboxylate, TcCLB.509207.90

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.2 pyrroline-5-carboxylate reductase

Purification

Purification on EC 1.5.1.2 - pyrroline-5-carboxylate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 isoenzymes
-
ammonium sulfate precipitation, anion exchange column chromatography, and gel filtration
-
by Ni-NTA His-binding resin affinity chromatography, the N-terminal fusion partner is cleaved by enterokinase
-
copurification with proline oxidase
-
copurification with proline oxidase, both activities located on the same protein
-
copurification with thiazolidine-4-carboxylate dehydrogenase
-
homogeneity
Ni+2-NTA His-Trap resin column chromatography, and gel filtration
-
nickel affinity gel column chromatography
-
partial
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3) pLysS by nickel affinity chromatography, dialysis, and ultrafiltration
using a HiTrap Ni-NTA column
using a Ni-NTA agarose, a Superdex200 and a Resource Q column
-
usung a Ni-NTA agarose, a Superdex-200 10/300 and a Resource Q column