1.5.1.2: pyrroline-5-carboxylate reductase

This is an abbreviated version!
For detailed information about pyrroline-5-carboxylate reductase, go to the full flat file.

Word Map on EC 1.5.1.2

Reaction

L-proline
+
NAD(P)+
=
1-pyrroline-5-carboxylate
+
NAD(P)H
+
H+

Synonyms

1-pyrroline-5-carboxylate reductase, DELTA1-pyrroline-5-carboxylate reductase, L-proline oxidase, L-proline-NAD(P)+ 5-oxidoreductase, L-proline:NAD(P)+ 5-oxidoreductase, MTR_7g090160, NADPH-L-DELTA'-pyrroline carboxylic acid reductase, P5C reductase, P5CDH, P5CR, PO, POX, proline oxidase, Pycr1, pyrroline-5-carboxylate dehydrogenase, pyrroline-5-carboxylate reductase, reductase, pyrroline-5-carboxylate

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.2 pyrroline-5-carboxylate reductase

Storage Stability

Storage Stability on EC 1.5.1.2 - pyrroline-5-carboxylate reductase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-10°C, 50% loss of activity in 4 weeks
-
-15°C, glutathione, EDTA
-
-18°C, 0.15-0.2 M potassium phosphate buffer pH 7.4, 2 months
-
-20°C, 1 mg protein/ml, 50% glycerol, 0.1 mM dithiothreitol, unstable
-
-20°C, 50% glycerol, 0.1 mM dithiothreitol, stable for 6 months
-
-20°C, inactivation in few days, unstable in the absence of NADPH
-
-70°C, 50 mM triethanolamine buffer pH 7.4, 9% w/v sucrose, 1 mM EDTA, 2 mM MgCl2, 30 mM 2-mercaptoethanol
-
-80°C, 1 year
-
-80°C, 20 mM Caps, pH 9.4, 0.5 M NaCl, 6 months
-
-80°C, 5% glycerol, 2 mM dithiothreitol, 1 month
-
3°C, 0.1 M sodium phosphate buffer pH 7.6, 10% (NH4)2SO4, 7 days
-
4°C, 50% loss of activity in 1 weeks
-