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4.6
10-formyl-dihydrofolate
pH 7.5, temperature not specified in the publication
0.003 - 284
7,8-dihydrofolate
2
acetylpyridine adenine nucleotide
-
with 7,8-dihydrofolate
35.9
NADP+
pH 7.8, 25°C, recombinant enzyme
additional information
additional information
-
0.003
7,8-dihydrofolate
pH 7, 20°C, mutant enzyme V11D
0.025
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31L
0.05
7,8-dihydrofolate
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.06
7,8-dihydrofolate
pH 7, 20°C, wild-type enzyme
0.2
7,8-dihydrofolate
-
mutant I51N/C59R/N108S/I164L, pH 7.0
0.22
7,8-dihydrofolate
deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C
0.243
7,8-dihydrofolate
-
altered enzyme
0.3
7,8-dihydrofolate
-
mutant enzyme R44P, at 15°C, pH not specified in the publication
0.37
7,8-dihydrofolate
deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C
0.38
7,8-dihydrofolate
deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C
0.49
7,8-dihydrofolate
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
0.5
7,8-dihydrofolate
mutant F31R/F34A/Q35N, pH 7.6, 23°C
0.65
7,8-dihydrofolate
-
recombinant enzyme
0.65
7,8-dihydrofolate
-
mutant V115C, pH 7.6, 23°C
0.69
7,8-dihydrofolate
-
mutant enzyme R44D, at 15°C, pH not specified in the publication
0.86
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31A
0.89
7,8-dihydrofolate
-
mutant enzyme R44E, at 15°C, pH not specified in the publication
0.89
7,8-dihydrofolate
-
mutant enzyme R44Y, at 15°C, pH not specified in the publication
0.95
7,8-dihydrofolate
-
-
0.98
7,8-dihydrofolate
-
mutant enzyme R44L, at 15°C, pH not specified in the publication
1
7,8-dihydrofolate
mutant F31R/F34T/Q35R, pH 7.6, 23°C
1
7,8-dihydrofolate
mutant F31R/F34T/Q35S, pH 7.6, 23°C
1.1
7,8-dihydrofolate
mutant F31A/F34V/Q35H, pH 7.6, 23°C
1.15
7,8-dihydrofolate
-
mutant enzyme R44I, at 15°C, pH not specified in the publication
1.23
7,8-dihydrofolate
-
mutant N51I/C59R/S108N, pH 8.0, 25°C
1.24
7,8-dihydrofolate
-
wild-type, pH 8.0, 25°C
1.3
7,8-dihydrofolate
mutant F31R/Q35E, pH 7.6, 23°C
1.31
7,8-dihydrofolate
-
mutant enzyme R44V, at 15°C, pH not specified in the publication
1.39
7,8-dihydrofolate
-
mutant enzyme R44F, at 15°C, pH not specified in the publication
1.39
7,8-dihydrofolate
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
1.4
7,8-dihydrofolate
-
above, pH 6.0, 25°C recombinant His-tagged enzyme
1.42
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C
1.5
7,8-dihydrofolate
-
pH 8.0, 25°C
1.5
7,8-dihydrofolate
-
mutant N51I/C59R/N108S/I164L, pH 7.0
1.5
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31Q
1.59
7,8-dihydrofolate
-
mutant enzyme R44H, at 15°C, pH not specified in the publication
1.6
7,8-dihydrofolate
-
with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5
1.7
7,8-dihydrofolate
mutant F31P/Q35E, pH 7.6, 23°C
1.7
7,8-dihydrofolate
mutant F31P/Q35H, pH 7.6, 23°C
1.7
7,8-dihydrofolate
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
1.7
7,8-dihydrofolate
deletion mutant DELTAGly121, at pH 7.0 and 25°C
1.7
7,8-dihydrofolate
mutant enzyme Y102F, at pH 7.0 and 25°C
1.78
7,8-dihydrofolate
-
mutant enzyme R44G, at 15°C, pH not specified in the publication
1.9
7,8-dihydrofolate
-
mutant N51I/C59R/N108T/I164L, pH 7.0
1.9
7,8-dihydrofolate
-
mutant enzyme R44Q, at 15°C, pH not specified in the publication
2 - 8
7,8-dihydrofolate
recombinant mutant N64F, pH 7.4, 37°C
2
7,8-dihydrofolate
-
with acetylpyridine adenine nucleotide
2
7,8-dihydrofolate
-
mutant enzyme R44T, at 15°C, pH not specified in the publication
2.2
7,8-dihydrofolate
-
pH 6, 25°C
2.3
7,8-dihydrofolate
-
with NAPH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5
2.41
7,8-dihydrofolate
-
mutant enzyme R44W, at 15°C, pH not specified in the publication
2.43
7,8-dihydrofolate
-
mutant enzyme R44C, at 15°C, pH not specified in the publication
2.7
7,8-dihydrofolate
-
pH 8.0, 25°C, presence of 500 mM NaCl
2.8
7,8-dihydrofolate
-
mutant V115A, pH 7.6, 23°C
3 - 6
7,8-dihydrofolate
recombinant wild-type enzyme, pH 7.4, 37°C
3 - 6
7,8-dihydrofolate
-
pH 7.4, 37°C, mutant K37Q
3 - 6
7,8-dihydrofolate
recombinant mutant N64S, pH 7.4, 37°C
3 - 6
7,8-dihydrofolate
recombinant mutant Q35K/N64S, pH 7.4, 37°C
3
7,8-dihydrofolate
-
isozyme I
3.05
7,8-dihydrofolate
-
mutant enzyme R44A, at 15°C, pH not specified in the publication
3.1
7,8-dihydrofolate
-
pH 8.0, 25°C, presence of 200 mM NaCl
3.11
7,8-dihydrofolate
-
wild type enzyme, in 50 mM TES, pH 7.0, at 37°C
3.14
7,8-dihydrofolate
-
recombinant wild-type enzyme
3.3
7,8-dihydrofolate
-
mutant enzyme R44N, at 15°C, pH not specified in the publication
3.4
7,8-dihydrofolate
-
pH 6.0, 25°C
3.58
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31E
3.75
7,8-dihydrofolate
-
mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C
3.8
7,8-dihydrofolate
-
mutant enzyme R44M, at 15°C, pH not specified in the publication
4
7,8-dihydrofolate
-
+ NADPH
4.3
7,8-dihydrofolate
mutant F31G/Q35N, pH 7.6, 23°C
4.54
7,8-dihydrofolate
-
mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C
4.6
7,8-dihydrofolate
mutant M1A/C85A/C152S, 15°C, pH 7.0
4.67
7,8-dihydrofolate
-
wild-type enzyme
4.8
7,8-dihydrofolate
mutant M1P/C85A/C152S, 15°C, pH 7.0
4.8
7,8-dihydrofolate
mutant enzyme F96I, at pH 7.0 and 25°C
4.9
7,8-dihydrofolate
mutant C85A/C152S, 15°C, pH 7.0
4.97
7,8-dihydrofolate
-
recombinant mutant A31K
5
7,8-dihydrofolate
-
pH 7.4
5
7,8-dihydrofolate
-
recombinant bifunctional enzyme
5.03
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31N
5.3
7,8-dihydrofolate
mutant M1S/C85A/C152S, 15°C, pH 7.0
5.3
7,8-dihydrofolate
wild-type, 15°C, pH 7.0
5.3
7,8-dihydrofolate
-
wild-type, 15°C, pH 7.0
5.4
7,8-dihydrofolate
-
wild type enzyme, at 15°C, pH not specified in the publication
5.7
7,8-dihydrofolate
-
mutant enzyme R44K, at 15°C, pH not specified in the publication
5.8
7,8-dihydrofolate
-
mutant enzyme R44S, at 15°C, pH not specified in the publication
5.9
7,8-dihydrofolate
-
at pH 7.0 and 5°C
6.4
7,8-dihydrofolate
mutant F31P, pH 7.6, 23°C
7
7,8-dihydrofolate
-
native enzyme
7.2
7,8-dihydrofolate
pH 7.0, 30°C, mutant L32D
7.2
7,8-dihydrofolate
pH 6.8, 23°C
7.4
7,8-dihydrofolate
pH 6, 25°C
7.6
7,8-dihydrofolate
-
mutant R53M enzyme
8.2
7,8-dihydrofolate
-
at pH 7.0 and 10°C
8.33
7,8-dihydrofolate
-
-
8.63
7,8-dihydrofolate
-
pH 6.0
9
7,8-dihydrofolate
chimera with Homo sapiens enzyme, pH 7, 25°C
9.3
7,8-dihydrofolate
wild type enzyme, at pH 7.0 and 25°C
9.4
7,8-dihydrofolate
mutant F31S/Q35E, pH 7.6, 23°C
9.5
7,8-dihydrofolate
-
recombinant double mutant L30K/A31K
10
7,8-dihydrofolate
-
wild-type, pH 7.6, 23°C
10
7,8-dihydrofolate
wild-type, pH 7.6, 23°C
10
7,8-dihydrofolate
chimera with Haemophilus influenzae enzyme, pH 7, 25°C
10.8
7,8-dihydrofolate
-
at pH 7.0 and 15°C
11.4
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L/I150V/N182I/N201D, pH 7.0
11.5
7,8-dihydrofolate
-
-
11.7
7,8-dihydrofolate
-
pH 6.0, 25°C, presence of 200 mM NaCl
12
7,8-dihydrofolate
-
recombinant mutant S108C
12.4
7,8-dihydrofolate
-
mutant M42I, pH 7.0, 25°C
12.6
7,8-dihydrofolate
-
pH 7, 20°C, wild-type enzyme
13
7,8-dihydrofolate
wild-type, pH 7, 25°C
13
7,8-dihydrofolate
-
mutant M42G, pH 7.0, 25°C
13.5
7,8-dihydrofolate
-
-
13.6
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L/D187A, pH 7.0
13.7
7,8-dihydrofolate
-
wild-type enzyme from plasmid
14
7,8-dihydrofolate
-
mutant M42A, pH 7.0, 25°C
14
7,8-dihydrofolate
chimera with Listeria grayi enzyme, pH 7, 25°C
14.8
7,8-dihydrofolate
-
at pH 7.0 and 20°C
15
7,8-dihydrofolate
-
mutant type
15.7
7,8-dihydrofolate
-
mutant M42V, pH 7.0, 25°C
15.9
7,8-dihydrofolate
-
mutant M42E, pH 7.0, 25°C
15.9
7,8-dihydrofolate
pH 6, temperature not specified in the publication, isolate PKNY146CSP
16.2
7,8-dihydrofolate
mutant M1A/M16S/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
16.2
7,8-dihydrofolate
pH 7.0, 30°C, mutant L32A
16.5
7,8-dihydrofolate
-
-
16.5
7,8-dihydrofolate
deletion mutant DELTAGly67, at pH 7.0 and 25°C
16.8
7,8-dihydrofolate
-
recombinant enzyme
17
7,8-dihydrofolate
pH 6.8, 23°C
18
7,8-dihydrofolate
-
with NADPH
18.7
7,8-dihydrofolate
-
mutant M42T, pH 7.0, 25°C
18.7
7,8-dihydrofolate
pH 7.0, 30°C, mutant V76A
18.7
7,8-dihydrofolate
-
at pH 7.0 and 25°C
18.9
7,8-dihydrofolate
-
mutant M42L, pH 7.0, 25°C
20.5
7,8-dihydrofolate
pH 7.0, 30°C, wild-type
20.7
7,8-dihydrofolate
-
pH 6.0, 25°C, presence of 500 mM NaCl
21
7,8-dihydrofolate
-
recombinant mutant S108A
21
7,8-dihydrofolate
pH 7.0, 30°C, mutant L32F
21
7,8-dihydrofolate
recombinant mutant Q35S/N64F, pH 7.4, 37°C
22.6
7,8-dihydrofolate
-
mutant M42S, pH 7.0, 25°C
22.8
7,8-dihydrofolate
pH 7.5, temperature not specified in the publication
23
7,8-dihydrofolate
-
recombinant mutant S108G
23
7,8-dihydrofolate
recombinant mutant Q35S/N64S, pH 7.4, 37°C
23.1
7,8-dihydrofolate
deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C
24
7,8-dihydrofolate
-
mutant M42P, pH 7.0, 25°C
24
7,8-dihydrofolate
deletion mutant DELTAAla145, at pH 7.0 and 25°C
24
7,8-dihydrofolate
recombinant mutant Q35S, pH 7.4, 37°C
24.3
7,8-dihydrofolate
-
mutant M42Q, pH 7.0, 25°C
24.6
7,8-dihydrofolate
-
mutant L20M from plasmid
24.6
7,8-dihydrofolate
-
wild-type, pH 7.0, 25°C
24.8
7,8-dihydrofolate
wild type enzyme, at pH 7.0 and 25°C
25.2
7,8-dihydrofolate
-
double mutant L16M/L20M from plasmid
25.3
7,8-dihydrofolate
-
at pH 7.0 and 30°C
25.8
7,8-dihydrofolate
mutant M1A/M16F/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
26
7,8-dihydrofolate
recombinant wild-type enzyme, pH 7.4, 37°C
26
7,8-dihydrofolate
recombinant mutant S37Q/S69F, pH 7.4, 37°C
26.6
7,8-dihydrofolate
-
mutant M42H, pH 7.0, 25°C
27
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35S/N64S
28.3
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L/K96N, pH 7.0
28.8
7,8-dihydrofolate
-
at pH 7.0 and 45°C
29
7,8-dihydrofolate
-
wild-type enzyme
29
7,8-dihydrofolate
chimera with Saccharomyces cerevisiae enzyme, pH 7, 25°C
30.4
7,8-dihydrofolate
-
mutant M42C, pH 7.0, 25°C
31.9
7,8-dihydrofolate
deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C
32.5
7,8-dihydrofolate
-
at pH 7.0 and 35°C
33
7,8-dihydrofolate
chimera with Plasmodium falciparum enzyme, pH 7, 25°C
34.8
7,8-dihydrofolate
mutant circular ANLYF-G8, 15°C, pH 7.0
35
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35S
36
7,8-dihydrofolate
-
recombinant mutant S108T
36.7
7,8-dihydrofolate
mutant linear ANLYF-G6, 15°C, pH 7.0
37
7,8-dihydrofolate
-
recombinant mutant S108Q
37
7,8-dihydrofolate
chimera with Streptococcus dysgalactiae enzyme, pH 7, 25°C
38
7,8-dihydrofolate
recombinant mutant K37Q, pH 7.4, 37°C
38.7
7,8-dihydrofolate
mutant circular ANLYF-G7, 15°C, pH 7.0
39
7,8-dihydrofolate
recombinant mutant Q35K, pH 7.4, 37°C
39.6
7,8-dihydrofolate
-
at pH 7.0 and 40°C
40
7,8-dihydrofolate
pH 7.4, 37°C, wild-type enzyme and mutant N64F
40.5
7,8-dihydrofolate
mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
40.5
7,8-dihydrofolate
mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, 15°C, pH 7.0
40.8
7,8-dihydrofolate
mutant linear ANLYF-G8, 15°C, pH 7.0
41.1
7,8-dihydrofolate
deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C
41.7
7,8-dihydrofolate
mutant linear ANLYF-G7, 15°C, pH 7.0
43
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35K/N64F
43
7,8-dihydrofolate
recombinant mutant Q35K/N64F, pH 7.4, 37°C
43.7
7,8-dihydrofolate
mutant circular ANLYF-G6, 15°C, pH 7.0
45
7,8-dihydrofolate
recombinant mutant K37S, pH 7.4, 37°C
45.5
7,8-dihydrofolate
-
mutant M42Y, pH 7.0, 25°C
47
7,8-dihydrofolate
pH 7.4, 37°C, mutant N64S
47
7,8-dihydrofolate
recombinant mutant S69N, pH 7.4, 37°C
48
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35K
51
7,8-dihydrofolate
recombinant mutant S37Q, pH 7.4, 37°C
52.7
7,8-dihydrofolate
-
triple mutant L16M/L20M/L42M from plasmid
53
7,8-dihydrofolate
-
pH 7.4, 37°C, wild-type enzyme
57
7,8-dihydrofolate
recombinant mutant S37K/S69F, pH 7.4, 37°C
72
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L, pH 7.0
78
7,8-dihydrofolate
recombinant mutant S37K/S69N, pH 7.4, 37°C
83
7,8-dihydrofolate
recombinant mutant S69F, pH 7.4, 37°C
88
7,8-dihydrofolate
-
recombinant wild-type enzyme
89
7,8-dihydrofolate
-
mutant M42W, pH 7.0, 25°C
92
7,8-dihydrofolate
-
recombinant mutant S108N
95
7,8-dihydrofolate
recombinant mutant S37Q/S69N, pH 7.4, 37°C
96.3
7,8-dihydrofolate
deletion mutant DELTAArg52, at pH 7.0 and 25°C
100
7,8-dihydrofolate
-
form I
100
7,8-dihydrofolate
-
recombinant mutant L30K
104.6
7,8-dihydrofolate
-
mutant M42W, pH 7.0, 25°C
108
7,8-dihydrofolate
-
recombinant enzyme
123
7,8-dihydrofolate
-
25°C, pH 7.4
130
7,8-dihydrofolate
pH 7.8, 25°C, recombinant enzyme
133
7,8-dihydrofolate
-
wild-type
157
7,8-dihydrofolate
recombinant mutant K37S/F69S, pH 7.4, 37°C
158
7,8-dihydrofolate
recombinant mutant K37Q/F69S, pH 7.4, 37°C
190
7,8-dihydrofolate
recombinant mutant K37S/F69N, pH 7.4, 37°C
204
7,8-dihydrofolate
recombinant mutant F69S, pH 7.4, 37°C
223
7,8-dihydrofolate
recombinant mutant K37Q/F69N, pH 7.4, 37°C
235
7,8-dihydrofolate
recombinant wild-type enzyme, pH 7.4, 37°C
284
7,8-dihydrofolate
-
pH 7.4, 37°C, wild-type enzyme
3.8
dihydrofolate
mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C
18.4
dihydrofolate
-
pH 7.0, 25°C, under atmospheric pressure
31.5
dihydrofolate
wild-type, pH 7.0, 25°C
51
dihydrofolate
pH 7.5, 25°C, cosubstrate: NADPH
83.8
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
90
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
144
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
156
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
0.02
folate
-
-
0.125
folate
-
mutant type
0.02
NADPH
pH 7.0, 30°C, mutant D31L
0.05
NADPH
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.59
NADPH
pH 7.0, 30°C, mutant D31A
1.39
NADPH
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
2 - 8
NADPH
recombinant mutant N64F, pH 7.4, 37°C
2
NADPH
-
pH 8.0, 25°C, presence of 1000 mM NaCl
2 - 3.7
NADPH
pH 7.0, 30°C, mutant V76A
2.05
NADPH
pH 7.0, 30°C, mutant D31E
2.18
NADPH
pH 7.0, 30°C, mutant D31Q
2.8
NADPH
-
mutant W48Y, 25°C, pH 7.0
2.8
NADPH
-
pH 8.0, 25°C, presence of 500 mM NaCl
3 - 6
NADPH
recombinant wild-type enzyme, pH 7.4, 37°C
3 - 6
NADPH
recombinant mutant N64S, pH 7.4, 37°C
3 - 6
NADPH
recombinant mutant Q35K/N64S, pH 7.4, 37°C
3.2
NADPH
-
pH 8.0, 25°C, presence of 200 mM NaCl
3.3
NADPH
-
mutant W48Y/N188Y, 25°C, pH 7.0
4.05
NADPH
pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate
8.5
NADPH
pH 7.0, 30°C, mutant D31N
8.5
NADPH
-
pH 6.0, 25°C, presence of 200 mM NaCl
10
NADPH
-
per binding site
10
NADPH
pH 7.0, 30°C, mutant L32D
11
NADPH
-
with 7,8-dihydrofolate
12.6
NADPH
-
pH 7, 20°C, wild-type enzyme
17.8
NADPH
pH 7.0, 30°C, mutant L32A
19.2
NADPH
-
pH 7.0, 25°C, under atmospheric pressure
20.3
NADPH
-
pH 6.0, 25°C, presence of 500 mM NaCl
21
NADPH
recombinant mutant Q35S/N64F, pH 7.4, 37°C
23
NADPH
recombinant mutant Q35S/N64S, pH 7.4, 37°C
23.2
NADPH
pH 7.0, 30°C, mutant L32F
24
NADPH
recombinant mutant Q35S, pH 7.4, 37°C
26
NADPH
recombinant wild-type enzyme, pH 7.4, 37°C
26
NADPH
recombinant mutant S37Q/S69F, pH 7.4, 37°C
26.8
NADPH
pH 7.0, 30°C, wild-type
28.1
NADPH
pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate
38
NADPH
recombinant mutant K37Q, pH 7.4, 37°C
39
NADPH
recombinant mutant Q35K, pH 7.4, 37°C
43
NADPH
recombinant mutant Q35K/N64F, pH 7.4, 37°C
45
NADPH
recombinant mutant K37S, pH 7.4, 37°C
47
NADPH
recombinant mutant S69N, pH 7.4, 37°C
51
NADPH
pH 7.5, 25°C, cosubstrate: dihydrofolate
51
NADPH
recombinant mutant S37Q, pH 7.4, 37°C
57
NADPH
recombinant mutant S37K/S69F, pH 7.4, 37°C
60.9
NADPH
-
wild-type, 25°C, pH 7.0
78
NADPH
recombinant mutant S37K/S69N, pH 7.4, 37°C
80.3
NADPH
pH 7.0, 25°C, under atmospheric pressure
83
NADPH
recombinant mutant S69F, pH 7.4, 37°C
86.8
NADPH
pH 7.0, 25°C, under atmospheric pressure
95
NADPH
recombinant mutant S37Q/S69N, pH 7.4, 37°C
109
NADPH
pH 7.0, 25°C, under atmospheric pressure
150.5
NADPH
pH 7.0, 25°C, under atmospheric pressure
157
NADPH
recombinant mutant K37S/F69S, pH 7.4, 37°C
158
NADPH
recombinant mutant K37Q/F69S, pH 7.4, 37°C
190
NADPH
recombinant mutant K37S/F69N, pH 7.4, 37°C
204
NADPH
recombinant mutant F69S, pH 7.4, 37°C
223
NADPH
recombinant mutant K37Q/F69N, pH 7.4, 37°C
235
NADPH
recombinant wild-type enzyme, pH 7.4, 37°C
additional information
additional information
-
-
-
additional information
additional information
-
selenomethionine-containing mutants
-
additional information
additional information
-
pyrimethamine sensitive and resistant strain
-
additional information
additional information
analysis of kinetic and thermodynamic fitting parameters and relaxation dispersion curves at different pH values and temperatures
-
additional information
additional information
-
analysis of kinetic and thermodynamic fitting parameters and relaxation dispersion curves at different pH values and temperatures
-
additional information
additional information
kcat for wild-type is pH-dependent, maximal kcat at pH 7.0
-
additional information
additional information
-
kcat for wild-type is pH-dependent, maximal kcat at pH 7.0
-