1.5.1.34: 6,7-dihydropteridine reductase
This is an abbreviated version!
For detailed information about 6,7-dihydropteridine reductase, go to the full flat file.
Word Map on EC 1.5.1.34
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1.5.1.34
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tetrahydrobiopterin
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hydroxylase
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bh4
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hyperphenylalaninemia
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neurotransmitter
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phenylketonuria
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biopterin
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pterins
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dopamine
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quinonoid
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sepiapterin
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1.6.99.7
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cyclohydrolase
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6-pyruvoyl-tetrahydropterin
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5-hydroxytryptophan
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tetrahydropterin
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dihydrobiopterin
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l-dopa
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ptp
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folinic
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nadh-specific
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homovanillic
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pterin-4a-carbinolamine
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guthrie
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6-pyruvoyl
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dihydropterins
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gtpch
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medicine
- 1.5.1.34
- tetrahydrobiopterin
- hydroxylase
- bh4
- hyperphenylalaninemia
-
neurotransmitter
- phenylketonuria
- biopterin
- pterins
- dopamine
-
quinonoid
- sepiapterin
-
1.6.99.7
-
cyclohydrolase
- 6-pyruvoyl-tetrahydropterin
- 5-hydroxytryptophan
- tetrahydropterin
- dihydrobiopterin
- l-dopa
- ptp
-
folinic
-
nadh-specific
-
homovanillic
-
pterin-4a-carbinolamine
-
guthrie
-
6-pyruvoyl
- dihydropterins
- gtpch
- medicine
Reaction
Synonyms
7,8-dihydrobiopterin reductase, BmDhpr, DHPR, dicDHPR, dihydropteridine reductase, dihydropteridine reductase (NADH), DQPR gene product, EC 1.6.99.10, EC 1.6.99.7, EcDHPR, More, NADH-dihydropteridine reductase, NADPH-dihydropteridine reductase, NADPH-specific dihydropteridine reductase, NfsB, NprA nitroreductase, PTR2, QDPR, quinoid dihydropteridine reductase, reductase, dihydropteridine (reduced nicotinamide adenine dinucleotide)
ECTree
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Purification
Purification on EC 1.5.1.34 - 6,7-dihydropteridine reductase
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cells centrifuged, washed with PBS (pH 7.4), resuspended in lysis buffer (50 mM Tris-HCl, pH 7.5), disrupted by sonication, centrifugation, supernatant filtered (0.45 microm), applied to a column with nickel-NTA beads, eluted (50 mM Tris-HCl, pH 7.5), active fractions pooled, concentrated, and exchanged into 50 mM sodium phosphate, pH 6.8, ultrafiltration, applied to a cation-exchange chromatography HS20 column, eluted with salt gradient at pH 6.8, concentration by ultrafiltration, gel-filtration chromatography with Superdex 200 column in 50 mM Tris-HCl, pH 8.0, concentration of eluted fractions in 20 mM Tris-HCl, pH 8.0, by ultrafiltration
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homogenization of smooth muscle strips in 2-DE lysis buffer containing 8 M urea, 2 M thiourea, 65 mM DTT, 2% CHAPS, and protease inhibitor cocktail, centrifugation, supernatant used for 2-DE and MALDI-TOF/TOF-MS
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native enzyme and recombinant wild-type, W104F, A6V and D37I mutant enzyme
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native enzyme from fifth larval instar of p50 and ah09 strains, recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and ultrafiltration
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recombinant enzyme from Escherichia coli by ion exchange chromatography and gel filtration to homogeneity
recombinant enzyme, partially from Escherichia coli, solubilization at pH 10.0
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneity
simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose
wild-type and recombinant Y150H, Y150S, Y150F, Y150E, Y150K, G151S and G23D mutant enzymes
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simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose
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simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose
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simple two step procedure i.e. affinity chromatography on Matrex gel blue A and hydrophobic chromatography on phenyl-Sepharose
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