1.5.1.38: FMN reductase (NADPH)

This is an abbreviated version!
For detailed information about FMN reductase (NADPH), go to the full flat file.

Reaction

Synonyms

(NADPH)-dependent flavin mononucleotide reductase, (NADPH)-dependent FMN reductase, BC_1619, EC 1.5.1.29, EC 1.6.8.1, flavin reductase P, FMN reductase, FRP, More, NAD(P)H:FMN reductase, NADPH specific FMN reductase, NADPH-flavin oxidoreductase, NADPH-FMN oxidoreductase, NADPH:FMN oxidoreductase, NADPH:FMN reductase, SsuE, ssueE, ycbP, ydgI

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.1 With NAD⁺ or NADP⁺ as acceptor

1.5.1.38 FMN reductase (NADPH)

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Results	in table
4073	AA Sequence
7	Cloned(Commentary)
16	Cofactor
3	Crystallization (Commentary)
11	General Information
9	Inhibitors
13	kcat/KM [mM/s]
28	KM Value [mM]
18	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
17	Organism
4	Pathway
31	PDB ID
8	pH Optimum
1	pH Range
10	Protein Variants
10	Purification (Commentary)
6	Reaction
17	Reference
1	Renatured (Commentary)
3	Specific Activity [micromol/min/mg]
2	Storage Stability
36	Substrates and Products (Substrate)
12	Subunits
42	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Stability [°C]
8	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 1.5.1.38 - FMN reductase (NADPH)

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23700

Escherichia coli

P80644

2 * 23700, calculated from sequence

25400

2 * 25400, SDS-PAGE

26312

1 * 26312, calculated from sequence

28000

1 * 28000, SDS-PAGE

39000

gel filtration, dimeric flavin-free mutant Y118A

40000

40900

dimeric SsueE in presence of flavin, analytical ultracentrifugation

741896

42000

Escherichia coli

P80644

gel filtration, dimeric flavin-bound mutants Y118A and Y118S

58400

gel filtration

60000

recombinant His-tagged enzyme, gel filtration

741865

63000

Vibrio harveyi

sucrose density gradient centrifugation

392179

73000

Escherichia coli

P80644

gel filtration, tetrameric flavin-bound wild-type enzyme

764152

73100

Escherichia coli

P80644

tetrameric SsuE enzyme in the absence of flavin, analytical ultracentrifugation

741896

79000

Escherichia coli

P80644

gel filtration, tetrameric flavin-free mutant Y118F

764152

84000

Escherichia coli

P80644

gel filtration, tetrameric flavin-free mutant DELTAY118

764152

additional information

Escherichia coli

formation of a stable complex between the flavin mononucleotide (FMN) reductase (SsuE) and monooxygenase (SsuD) of the alkanesulfonate monooxygenase system. The stoichiometry for protein-protein interactions is proposed to involve a 1:1 monomeric association of SsuE with SsuD. Interactions between the two proteins do not lead to overall conformational changes in protein structure

674310