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1.5.1.38: FMN reductase (NADPH)

This is an abbreviated version!
For detailed information about FMN reductase (NADPH), go to the full flat file.

Reaction

FMNH2
+
NADP+
=
FMN
+
NADPH
+
H+

Synonyms

(NADPH)-dependent flavin mononucleotide reductase, (NADPH)-dependent FMN reductase, BC_1619, EC 1.5.1.29, EC 1.6.8.1, flavin reductase P, FMN reductase, FRP, More, NAD(P)H:FMN reductase, NADPH specific FMN reductase, NADPH-flavin oxidoreductase, NADPH-FMN oxidoreductase, NADPH:FMN oxidoreductase, NADPH:FMN reductase, SsuE, ssueE, ycbP, ydgI

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.38 FMN reductase (NADPH)

Renatured

Renatured on EC 1.5.1.38 - FMN reductase (NADPH)

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
insolubleoverexpressed enzyme protein is resuspended in a resuspension buffer containing 20 ml, 20 mM Tris-HCL, 0.5 M NaCl, pH 8.0, sonicated for 50 s discontinuously, spun down at 8000 rpm (5400 x g) at 4°C for 10 min, pellets resuspended in isolation buffer containing 15 ml, 20 mM Tris-HCL, 0.5 M NaCl, 2% Triton X-100, and 12% w/v urea, pH 8.0