1.5.1.40: 8-hydroxy-5-deazaflavin:NADPH oxidoreductase

This is an abbreviated version!
For detailed information about 8-hydroxy-5-deazaflavin:NADPH oxidoreductase, go to the full flat file.

Reaction

Synonyms

5-deazaflavin-NADP+ reductase, 8-hydroxy-5-deazaflavin-dependent NADP+ reductase, 8-OH-5-deazaflavin:NADPH oxidoreductase, 8-OH-5dFl:NADPH oxidoreductase, AF0892, F420-dependent NADP oxidoreductase, F420-dependent NADP reductase, F420-dependent NADP+ oxidoreductase, F420:NADPH oxidoreductase, F420H2:NADP oxidoreductase, F420H2:NADP+ oxidoreductase, Fno, Msm_0049, NADP+:F420 oxidoreductase, Tfu-FNO, Tfu_0970

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.40 8-hydroxy-5-deazaflavin:NADPH oxidoreductase

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Results	in table
94	AA Sequence
1	Application
5	Cloned(Commentary)
10	Cofactor
2	Crystallization (Commentary)
16	General Information
3	General Stability
23	Inhibitors
65	kcat/KM [mM/s]
9	Ki Value [mM]
106	KM Value [mM]
2	Metals/Ions
16	Molecular Weight [Da]
15	Natural Substrates/ Products (Substrates)
2	Organic Solvent Stability
44	Organism
1	Oxidation Stability
21	pH Optimum
4	pH Range
1	pI Value
29	Protein Variants
10	Purification (Commentary)
1	Reaction
36	Reference
6	Source Tissue
9	Specific Activity [micromol/min/mg]
6	Storage Stability
57	Substrates and Products (Substrate)
12	Subunits
47	Synonyms
1	Systematic Name
16	Temperature Optimum [°C]
3	Temperature Range [°C]
2	Temperature Stability [°C]
71	Turnover Number [1/s]

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Application on EC 1.5.1.40 - 8-hydroxy-5-deazaflavin:NADPH oxidoreductase

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APPLICATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

synthesis

Thermobifida fusca

Q47RA9

Tfu-FNO variants can be used as NCB-Recycling systems for TsOYE-mediated conversions. TsOYE is known to efficiently hydrogenate ketoisophorone (2,6,6-trimethyl-2-cyclohexene-1,4-dione) to form the chiral product (R)-2,2,6- trimethylcyclohexane-1,4-dione with high enantiomeric excess values of 94-97%, when several NCBs as more efficient electron donors in comparison to NADPH are used. The Tfu-FNO variants G29Y, G29W, and P89H, which show high activity toward 1-aminoethylnicotinamide (AmNA+), 1-benzylnicotinamide (BNA+), and 1-benzyl-3-acetylpyridine (BAP+), are selected for use in conversion experiments as recycling catalysts for these NCBs. Potential use of these thermostable Tfu- FNO variants as NCB recycling systems at high temperatures

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