1.5.1.54: methylenetetrahydrofolate reductase (NADH)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase (NADH), go to the full flat file.

Reaction

Synonyms

5,10-CH2-H4folate reductase, 5,10-methylenetetrahydrofolate reductase, 5,10-methylenetetrahydrofolate reductase (FADH2), 5,10-methylenetetrahydrofolic acid reductase, 5,10-methylenetetrahydropteroylglutamate reductase, 5-methyltetrahydrofolate:(acceptor) oxidoreductase, metF, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase, methylenetetrahydrofolate reductase, methylenetetrahydrofolic acid reductase, MSMEG_6596, MSMEG_6649, MTHFR1, MTHFR2, N5,10-methylenetetrahydrofolate reductase, N5,N10-methylenetetrahydrofolate reductase

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.1 With NAD⁺ or NADP⁺ as acceptor

1.5.1.54 methylenetetrahydrofolate reductase (NADH)

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All data fields related to EC 1.5.1.54
Results	in table
3	Activating Compound
8	AA Sequence
1	Application
3	Cloned(Commentary)
13	Cofactor
5	Crystallization (Commentary)
5	General Information
1	General Stability
7	Inhibitors
4	kcat/KM [mM/s]
6	Ki Value [mM]
34	KM Value [mM]
2	Localization
1	Metals/Ions
7	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
23	Organism
8	Pathway
13	Protein Variants
3	Purification (Commentary)
1	Reaction
21	Reference
3	Specific Activity [micromol/min/mg]
1	Storage Stability
38	Substrates and Products (Substrate)
11	Subunits
25	Synonyms
1	Systematic Name
2	Temperature Stability [°C]
19	Turnover Number [1/s]

Application

Application on EC 1.5.1.54 - methylenetetrahydrofolate reductase (NADH)

for references in articles please use BRENDA:EC1.5.1.54

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synthesis

Lactococcus lactis subsp. lactis

A0A0B8QP67

improvement of the biosynthesis of 5-MTHF by Lactococcus lactis by systematic pathway engineering, including the overexpression of the key rate-limiting enzyme MTHFR, strengthening 5-MTHF biosynthesis and folate supply by coexpression of the drfA, metF, and folE genes, increasing NADPH supply by overexpression of G6PDH, and increasing the proportion of 5-MTHF in various folates by overexpressing 5-formyltetrahydrofolate cyclo-ligase. The 5-MTHF titer finally reaches 300 microg/l with key precursor addition

761689