1.5.1.54: methylenetetrahydrofolate reductase (NADH)

This is an abbreviated version!
For detailed information about methylenetetrahydrofolate reductase (NADH), go to the full flat file.

Reaction

Synonyms

5,10-CH2-H4folate reductase, 5,10-methylenetetrahydrofolate reductase, 5,10-methylenetetrahydrofolate reductase (FADH2), 5,10-methylenetetrahydrofolic acid reductase, 5,10-methylenetetrahydropteroylglutamate reductase, 5-methyltetrahydrofolate:(acceptor) oxidoreductase, metF, methylenetetrahydrofolate (reduced riboflavin adenine dinucleotide) reductase, methylenetetrahydrofolate reductase, methylenetetrahydrofolic acid reductase, MSMEG_6596, MSMEG_6649, MTHFR1, MTHFR2, N5,10-methylenetetrahydrofolate reductase, N5,N10-methylenetetrahydrofolate reductase

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.1 With NAD⁺ or NADP⁺ as acceptor

                1.5.1.54 methylenetetrahydrofolate reductase (NADH)

Advanced search results

Do not include text mining results

Include results (more...)

Include results (more...)

Results	in table
3	Activating Compound
8	AA Sequence
1	Application
3	Cloned(Commentary)
13	Cofactor
5	Crystallization (Commentary)
5	General Information
1	General Stability
7	Inhibitors
4	kcat/KM [mM/s]
6	Ki Value [mM]
34	KM Value [mM]
2	Localization
1	Metals/Ions
7	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
23	Organism
8	Pathway
13	Protein Variants
3	Purification (Commentary)
1	Reaction
21	Reference
3	Specific Activity [micromol/min/mg]
1	Storage Stability
38	Substrates and Products (Substrate)
11	Subunits
25	Synonyms
1	Systematic Name
2	Temperature Stability [°C]
19	Turnover Number [1/s]

KM Value

print visible entries

print all entries

KM Value on EC 1.5.1.54 - methylenetetrahydrofolate reductase (NADH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.

top print hide show all columns Go to KM Value Search

Please wait a moment until the data is sorted. This message will disappear when the data is sorted.

KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0004 - 0.287

5,10-methylenetetrahydrofolate

13 entries

0.075 - 0.106

5-methyltetrahydrofolate

3 entries

0.0035 - 0.873

NADH

18 entries

0.0004

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

wild-type, pH 7.2, 4°C

760434

0.0005

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

wild-type, cosubstrate NADH, pH 7.2, 25°C

760563

0.0008

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

pH 7.2, 15°C

761372

0.0039

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

mutant A177V, pH 7.2, 15°C

762001

0.0039

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

wild-type, pH 7.2, 15°C

762001

0.027

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

mutant D120N, cosubstrate NADH, pH 7.2, 25°C

760563

0.0632

5,10-methylenetetrahydrofolate

Mycolicibacterium smegmatis

A0R6M0, A0R6S0

pH 7.2, 23°C

761402

0.104

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

mutant Q183A, pH 7.2, 4°C

760434

0.106

5,10-methylenetetrahydrofolate

Arabidopsis thaliana

Q9SE60

Chimera-1, cosubstrate NADPH, pH 7.2, temperature not specified in the publication

761431

0.108

5,10-methylenetetrahydrofolate

Escherichia coli

P0AEZ1

mutant Q183E, pH 7.2, 4°C

760434

0.145

5,10-methylenetetrahydrofolate

Mycolicibacterium smegmatis

A0R6M0, A0R6S0

pH 7.2, 23°C

761402

0.152

5,10-methylenetetrahydrofolate

Arabidopsis thaliana

Q9SE60

Chimera-1, cosubstrate NADH, pH 7.2, temperature not specified in the publication

761431

0.287

5,10-methylenetetrahydrofolate

Arabidopsis thaliana

Q9SE60

wild-type, cosubstrate NADH, pH 7.2, temperature not specified in the publication

761431

0.075

5-methyltetrahydrofolate

Escherichia coli

P0AEZ1

pH 7.2, 15°C

761372

0.085

5-methyltetrahydrofolate

Escherichia coli

P0AEZ1

wild-type, cosubstrate menadione, pH 7.2, 25°C

760563

0.106

5-methyltetrahydrofolate

Escherichia coli

P0AEZ1

mutant D120N, cosubstrate menadione, pH 7.2, 25°C

760563

0.0035

NADH

Escherichia coli

P0AEZ1

wild-type, pH 7.2, 4°C

760434

0.005

NADH

Escherichia coli

P0AEZ1

mutant D120N, pH 7.2, 25°C

760563

0.0066

NADH

Escherichia coli

P0AEZ1

mutant Q183E, pH 7.2, 4°C

760434

0.01

NADH

Blautia producta

-

pH 5.5, 37°C

761018

0.01

NADH

Escherichia coli

P0AEZ1

mutant Q183A, pH 7.2, 4°C

760434

0.013

NADH

Escherichia coli

P0AEZ1

pH 7.2, 15°C

761372

0.017

NADH

Escherichia coli

P0AEZ1

mutant A177V, pH 7.2, 15°C

762001

0.017

NADH

Escherichia coli

P0AEZ1

mutant D120N, cosubstrate menadione, pH 7.2, 25°C

760563

0.017

NADH

Escherichia coli

P0AEZ1

wild-type, pH 7.2, 15°C

762001

0.02

NADH

Escherichia coli

P0AEZ1

wild-type, pH 7.2, 25°C

760563

0.0325

NADH

Mycolicibacterium smegmatis

A0R6M0, A0R6S0

pH 7.2, 23°C

761402

0.066

NADH

Escherichia coli

P0AEZ1

wild-type, cosubstrate menadione, pH 7.2, 25°C

760563

0.08

NADH

Escherichia coli

P0AEZ1

mutant E28Q, cosubstrate menadione, pH 7.2, 25°C

760563

0.108

NADH

Mycolicibacterium smegmatis

A0R6M0, A0R6S0

pH 7.2, 23°C

761402

0.175

NADH

Escherichia coli

P0AEZ1

pH 7.2, 37°C

760473

0.24

NADH

Escherichia coli

P0AEZ1

presence of 0.05 mM genistein, pH 7.2, 37°C

760473

0.25

NADH

Escherichia coli

P0AEZ1

presence of 0.1 mM genistein, pH 7.2, 37°C

760473

0.873

NADH

Arabidopsis thaliana

Q9SE60

wild-type, pH 7.2, temperature not specified in the publication

761431