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1.5.1.B5: DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH)

This is an abbreviated version!
For detailed information about DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH), go to the full flat file.

Wordmap for 1.5.1.B5

Word Map on EC 1.5.1.B5 Wordmap for 1.5.1.B5

Reaction

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L-pipecolate
+
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NAD+
=
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DELTA1-piperideine-2-carboxylate
+
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NADH
+
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H+

Synonyms

DELTA-pyrroline-2-carboxylate reductase, DELTA1-pyrroline-2-carboxylate reductase, NADH-dependent Pyr2C reductase, Pyr2C reductase, TlLhpI, tlPyr2C

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                EC 1.5.1.B51.5.1.B5 DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH)

Cloned

Cloned on EC 1.5.1.B5 - DELTA1-piperideine-2-carboxylate/DELTA1-pyrroline-2-carboxylate reductase (NADH)

for references in articles please use BRENDA:EC1.5.1.B5

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
gene lphI, sequence comparisons and phylogenetic analysis, recombinant expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)-RIL
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His-tagged protein is expressed in Escherichia coli BL21. TlLhpI is a fusion of OCC_00362 and OCC_00367 genes by PCR. The gene from Thermococcus litoralis DSM 5473 consists of two separate open-reading frames (ORFs): OCC_00362 and OCC_00367. When a single A is inserted between A227646 and T227647 in the original genome sequence of T. litoralis DSM 5473, the separate ORFs fuse into a single ORF, and the putative amino acid sequence of is homologous to that of the Thermococcus sibiricus TSIB_0634 gene. Although there is a possibility that this OCD-like protein from Thermococcus litoralis DSM 5473 forms a heterooligomeric structure, the TlLhpI gene is synthesized as a single ORF by sequential steps of PCR using sense and antisense primers and genome DNA of Thermococcus litoralis DSM 5473 as a template
H3ZMH3; H3ZMH4