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1.5.3.1: sarcosine oxidase (formaldehyde-forming)

This is an abbreviated version!
For detailed information about sarcosine oxidase (formaldehyde-forming), go to the full flat file.

Word Map on EC 1.5.3.1

Reaction

sarcosine
+
H2O
+
O2
=
glycine
+
formaldehyde
+
H2O2

Synonyms

heterotetrameric sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase, monomeric sarcosine oxidase, MSOX, PSO, sarcosine : oxygen oxidoreductase (demethylating), sarcosine oxidase, sarcosine: O2 oxidoreductase, sarcosine:oxygen oxidoreductase (demethylating), SO, SO-U96, SOX, SoxA, trd_1773, TSOX

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.1 sarcosine oxidase (formaldehyde-forming)

Renatured

Renatured on EC 1.5.3.1 - sarcosine oxidase (formaldehyde-forming)

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD
-
reconstitution of holoprotein from inclusion bodies, suitable conditions for binding of flavoprotein to apoprotein are 0.3 g/l protein concentration, pH 8.5, 4°C, ratio of GSH/GSSH 2
-