1.5.3.1: sarcosine oxidase (formaldehyde-forming)
This is an abbreviated version!
For detailed information about sarcosine oxidase (formaldehyde-forming), go to the full flat file.
Word Map on EC 1.5.3.1
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1.5.3.1
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flavin
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fad
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heterotetrameric
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n-methylglycine
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creatininase
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amidinohydrolase
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n-methyltryptophan
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corynebacterial
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flavinylation
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diagnostics
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analysis
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medicine
- 1.5.3.1
- flavin
- fad
-
heterotetrameric
- n-methylglycine
- creatininase
-
amidinohydrolase
- n-methyltryptophan
-
corynebacterial
-
flavinylation
- diagnostics
- analysis
- medicine
Reaction
Synonyms
heterotetrameric sarcosine oxidase, L-pipecolate oxidase, L-pipecolic acid oxidase, monomeric sarcosine oxidase, MSOX, PSO, sarcosine : oxygen oxidoreductase (demethylating), sarcosine oxidase, sarcosine: O2 oxidoreductase, sarcosine:oxygen oxidoreductase (demethylating), SO, SO-U96, SOX, SoxA, trd_1773, TSOX
ECTree
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Renatured on EC 1.5.3.1 - sarcosine oxidase (formaldehyde-forming)
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production of soluble apoenzyme lacking FAD by controlled expression in Escherichia coli. Reconstitution of enzyme by incubation with FAD. Autoflavinylation occurs in a reaction that proceeds via reduced flavin intermediate and requires only apoenzyme and FAD
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reconstitution of holoprotein from inclusion bodies, suitable conditions for binding of flavoprotein to apoprotein are 0.3 g/l protein concentration, pH 8.5, 4°C, ratio of GSH/GSSH 2
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