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1.5.3.17: non-specific polyamine oxidase

This is an abbreviated version!
For detailed information about non-specific polyamine oxidase, go to the full flat file.

Word Map on EC 1.5.3.17

Reaction

N1-acetylspermidine
+
O2
 +
H2O
=
putrescine
 +
3-acetamidopropanal
 +
H2O2

Synonyms

AtPAO2, AtPAO3, AtPAO4, AtPAO5, Bjpao1, Bjpao2, EC 1.5.3.11, Fms1, LC036642, OsPAO1, PAO, PAO1, PAO2, PAO3, PAO5, polyamine oxidase 1, SelPAO5, slr5093, T-Spm oxidase, thermospermine oxidase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.17 non-specific polyamine oxidase

Cloned

Cloned on EC 1.5.3.17 - non-specific polyamine oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AtPAO2, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method
AtPAO3, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, recombinant expression as maltose-binding protein fusion protein in Escherichia coli, and expression of GFP-tagged AtPAO3 in transgenic Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method resulting in increased putrescine levels after feeding of exogenous spermidine
AtPAO3, expression as GFP-tagged enzyme in plant cells, expression of AtPAO3 fused to the maltose-binding protein in Escherichia coli strain BL21 in a temperature-dependent manner, overview
AtPAO4, DNA and amino acid sequence determination and analysis, intron/exon organization, sequence comparisons, expression of His6-tagged enzyme in Escherichia coli, expression of GFP-tagged AtPAO3 in Arabidopsis thaliana plants using the Agrobacterium tumefaciens (strain C58C1)-mediated floral dip transformation method
DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and mutant enzymes
-
expression in Escherichia coli
gene AtPAO2, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants
gene AtPAO3, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants
gene AtPAO5, construction of AtPAO::GUS transgenic Arabidopsis thaliana plants
production of recombinant SelPAO5 protein in Escherichia coli
recombinant expression of His-tagged wild-type and mutant enzymes
recombinant expression of wild-type and mutant enzymes
transient expression of isozyme AtPAO3 in Arabidopsis thaliana root cell peroxisomes as monomeric red fluorescent protein fusion protein
wild-type and mutant enzymes expressed in Escherichia coli