1.5.99.14: 6-hydroxypseudooxynicotine dehydrogenase

This is an abbreviated version!
For detailed information about 6-hydroxypseudooxynicotine dehydrogenase, go to the full flat file.

Reaction

Synonyms

KdhA, KdhB, KdhC, KdhL, KdhM, KdhS, PNO

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.99 With unknown physiological acceptors

                1.5.99.14 6-hydroxypseudooxynicotine dehydrogenase

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All data fields related to EC 1.5.99.14

Results	in table
254	AA Sequence
2	Cloned(Commentary)
7	Cofactor
1	Crystallization (Commentary)
4	General Information
10	kcat/KM [mM/s]
10	KM Value [mM]
2	Metals/Ions
4	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
21	Organism
4	Pathway
6	PDB ID
5	Protein Variants
1	Purification (Commentary)
1	Reaction
9	Reference
1	Specific Activity [micromol/min/mg]
10	Substrates and Products (Substrate)
3	Subunits
9	Synonyms
1	Systematic Name
10	Turnover Number [1/s]

General Information

General Information on EC 1.5.99.14 - 6-hydroxypseudooxynicotine dehydrogenase

for references in articles please use BRENDA:EC1.5.99.14

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GENERAL INFORMATION

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

physiological function

4 entries

physiological function

Agrobacterium tumefaciens

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6-hydroxypseudooxynicotine dehydrogenase utilizes the electron transfer flavoprotein EtfAB as the physiological electron acceptor. The electrons are further transferred from the reduced EtfAB to coenzyme Q by the catalysis of electron transfer flavoprotein:ubiquinone oxidoreductase. Disruption of the EtfAB genes in the nicotine-degrading gene cluster decreases the growth rate of Agrobacter tumefaciens S33 on nicotine but not on 6-hydroxy-3-succinoylpyridine

763956

physiological function

Agrobacterium tumefaciens

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Rid protein (participating in metabolite damage repair) encoded by a gene in the nicotine-degrading gene cluster enhances the reaction of 6-hydroxypseudooxynicotine dehydrogenase. Rid relieves the toxicity of the presumed imine intermediate produced in the 6-hydroxypseudooxynicotine dehydrogenase reaction and, in the presence of Rid, 6-hydroxypseudooxynicotine dehydrogenase maintains a high level of activity and the amount of the reaction product is increased by at least 5fold. Disruption of the Rid gene leads to slower growth on nicotine

763981

physiological function

Agrobacterium tumefaciens S33

-

6-hydroxypseudooxynicotine dehydrogenase utilizes the electron transfer flavoprotein EtfAB as the physiological electron acceptor. The electrons are further transferred from the reduced EtfAB to coenzyme Q by the catalysis of electron transfer flavoprotein:ubiquinone oxidoreductase. Disruption of the EtfAB genes in the nicotine-degrading gene cluster decreases the growth rate of Agrobacter tumefaciens S33 on nicotine but not on 6-hydroxy-3-succinoylpyridine

-

physiological function

Agrobacterium tumefaciens S33

-

Rid protein (participating in metabolite damage repair) encoded by a gene in the nicotine-degrading gene cluster enhances the reaction of 6-hydroxypseudooxynicotine dehydrogenase. Rid relieves the toxicity of the presumed imine intermediate produced in the 6-hydroxypseudooxynicotine dehydrogenase reaction and, in the presence of Rid, 6-hydroxypseudooxynicotine dehydrogenase maintains a high level of activity and the amount of the reaction product is increased by at least 5fold. Disruption of the Rid gene leads to slower growth on nicotine

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