1.5.99.15: dihydromethanopterin reductase (acceptor)

This is an abbreviated version!
For detailed information about dihydromethanopterin reductase (acceptor), go to the full flat file.

Reaction

5,6,7,8-tetrahydromethanopterin
+
oxidized acceptor
=
7,8-dihydromethanopterin
+
reduced acceptor

Synonyms

AfpA, archaeal-flavoprotein-like flavoprotein, dihydromethanopterin reductase, Dmr, DmrB, DmrX, H2MPT reductase, methylene H4MPT dehydrogenase B, MJ0208, MM1854, MtdB

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                1.5.99.15 dihydromethanopterin reductase (acceptor)

Crystallization

Crystallization on EC 1.5.99.15 - dihydromethanopterin reductase (acceptor)

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
hanging drop vapor diffusion method, using 0.8-1.2 M ammonium sulfate as precipitant
purified recombinant N-terminally His6 -tagged enzyme by hanging drop vapor diffusion method, mixing of 0.003 ml of 13 mg/ml protein in 20 mM Tris-HCl, pH 8.0, 100 mM NaCl, 5% glycerol, and 4 mM DTT, with reservori solution containing FMN, method optimization, 7-14 days at room temperature, X-ray diffraction structure determination and analysis at 1.9 A resolution