1.5.99.4: nicotine dehydrogenase

This is an abbreviated version!
For detailed information about nicotine dehydrogenase, go to the full flat file.

Word Map on EC 1.5.99.4

Reaction

(S)-nicotine
+
acceptor
+
H2O
=
(S)-6-hydroxynicotine
+
reduced acceptor

Synonyms

6-hydroxy-D-nicotine oxidase, 6-hydroxy-L-nicotine oxidase, D-nicotine oxidase, NctA, NDH, NdhAB, nicotine dehydrogenase, nicotine oxidase, nicotinic acid hydroxylase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.99 With unknown physiological acceptors
                1.5.99.4 nicotine dehydrogenase

Purification

Purification on EC 1.5.99.4 - nicotine dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, heat denaturation, TEAE-cellulose chromatography
-
ammonium sulfate precipitation, ion-exchange, gel filtration
-
ammonium sulfate precipitation, phenyl Sepharose column chromatography, DEAE-Sepharose column chromatography, and Superdex 200 gel filtration
-
hydroxylapatite column
-
protamine sulfate and ammonium sulfate precipitation, gel filtration, ion-exchange
-