1.5.99.B2: proline dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about proline dehydrogenase (acceptor), go to the full flat file.
Word Map on EC 1.5.99.B2
Reaction
Synonyms
APE_1267.1, dye-linked L-proline dehydrogenase, L-proDH, L-proline dehydrogenase, L-proline: FAD oxidoreductase, L-proline:FAD oxidoreductase, LPDH, PDH, PDH1, PDH2, PF1246, PF1798, PH1364, PH1751, PIG6, POX, Pro dehydrogenase, PRODH, ProDH1, ProDH2, proline dehydrogenase, proline oxidase, prub, PutA, TK0117, TK0122, TPpdhB, TPpdhB2, TtProDH
ECTree
1.5.99.B2 proline dehydrogenase (acceptor)Advanced search results
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results (Engineering
Engineering on EC 1.5.99.B2 - proline dehydrogenase (acceptor)
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PROTEIN VARIANTS 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
Y203F
site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
Y203F
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site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme
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H225A
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the mutant is unstable and precipitates from solution below pH 7.0, decreased activity compared to the wild type enzyme
H225Q
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the mutant displays activity down to solution pH 6.0, increased activity compared to the wild type enzyme
Y251F
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wild type pH stability, increased activity compared to the wild type enzyme
F10E/L12E
two point mutations in helix alphaA. This helix contains several hydrophobic residues. The recombinant MBP-tagged mutant variant exclusively forms tetramers and exhibits excellent catalytic features over a wide range of temperatures
additional information
additional information
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plants with altered levels of enzyme by sense and antisense strategies
additional information
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mutant plants lacking DELTA1 pyrroline-5-carboxylate dehydrogenase activate ProDH during hypersensitive response like the wild-type plants. They achieve maximum oxidative burst and cell death levels producing normal hypersensitive response lesions, but evidence premature defense activation
additional information
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transgenic plants bearing an antisense suppressor of enzyme have elevated salt tolerance and increased resistance to toxic proline analogue L-azetidine-2-carboxylic acid
