1.5.99.B2: proline dehydrogenase (acceptor)
This is an abbreviated version!
For detailed information about proline dehydrogenase (acceptor), go to the full flat file.
Word Map on EC 1.5.99.B2
Reaction
Synonyms
APE_1267.1, dye-linked L-proline dehydrogenase, L-proDH, L-proline dehydrogenase, L-proline: FAD oxidoreductase, L-proline:FAD oxidoreductase, LPDH, PDH, PDH1, PDH2, PF1246, PF1798, PH1364, PH1751, PIG6, POX, Pro dehydrogenase, PRODH, ProDH1, ProDH2, proline dehydrogenase, proline oxidase, prub, PutA, TK0117, TK0122, TPpdhB, TPpdhB2, TtProDH
ECTree
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results (Temperature Stability
Temperature Stability on EC 1.5.99.B2 - proline dehydrogenase (acceptor)
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TEMPERATURE STABILITY 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
100
100 - 105
-
the enzyme retains more than 90% of its activity after incubation at 100°C for 120 min (pH 7.5). The half-life at 105°C is about 90 min
30 - 70
-
the enzyme loses a considerable amount of its original activity at above 30°C and is nearly inactivated at 70°C
45
the enzyme loses 70% of activity upon incubation at 45°C for 15 min
70
90
100
-
no loss of activity being evident up to 100°C, above this temperature, thermal denaturation of PRODH is apparent, with complete loss of activity after 10 min of incubation in glycerol buffer at temperatures above 115°C
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase retains 100% activity under the same conditions
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase nearly loses all of its activity under the same conditions
70
-
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 76% activity under the same conditions
70
the alpha4beta4-type beta subunit of L-proline dehydrogenase retains more than 80% of activity after heating at 70°C for 20 min, while the alphabetagammadelta-type beta subunit of L-proline dehydrogenase shows 68% activity under the same conditions
90
PRODH exhibits over 85% residual activity after a 1 h incubation at 90°C and over 60% residual activity after 3 h
