1.6.2.4: NADPH-hemoprotein reductase

This is an abbreviated version!
For detailed information about NADPH-hemoprotein reductase, go to the full flat file.

Word Map on EC 1.6.2.4

Reaction

NADPH
+
H+
+ n oxidized hemoprotein =
NADP+
+ n reduced hemoprotein

Synonyms

aldehyde reductase (NADPH-dependent), ATR2, CPR, CPR I, CPR-X1, CPR-X2, CPR1, CPR2, CYPOR, cytochrome c reductase (reduced nicotinamide adenine dinucleotide phosphate, NADPH, NADPH-dependent), cytochrome P450 NADPH reductase, cytochrome P450 oxidoreductase, cytochrome P450 reductase, cytochrome P450-diflavin reductase, dihydroxynicotinamide adenine dinucleotide phosphate-cytochrome c reductase, EC 1.6.99.2, FAD-cytochrome c reductase, ferrihemprotein P450 reductase, flavocytochrome P450 BM3, NAD(P)H cytochrome P450 reductase, NADP-cytochrome c reductase, NADP-cytochrome P-450 reductase, NADP-cytochrome reductase, NADPH cytochrome P450 reductase, NADPH dependent cytochrome P450 reductase, NADPH P450 oxidoreductase, NADPH P450 reductase, NADPH-CPR, NADPH-CYP reductase, NADPH-CYP450 reductase, NADPH-cytochrome c oxidoreductase, NADPH-cytochrome c reductase, NADPH-cytochrome p-450 reductase, NADPH-cytochrome P450 (CYP) oxidoreductase, NADPH-cytochrome P450 oxidoreductase, NADPH-cytochrome P450 reductase, NADPH-cytochrome P450 reductase 2, NADPH-dependent cytochrome c reductase, NADPH-dependent cytochrome P450 reductase, NADPH-dependent P450 reductase, NADPH-ferricytochrome c oxidoreductase, NADPH-ferrihemoprotein reductase, NADPH-P450 reductase, NADPH: cytochrome P450 oxidoreductase, NADPH: cytochrome P450 reductase, NADPH:CYP oxidoreductase, NADPH:cytochrome P450 oxidoreductase, NADPH:cytochrome P450 reductase, NADPH:ferrihemoprotein oxidoreductase, neotetrazolin reductase, NPR, P-450 reductase, P450 BM3, P450 oxidoreductase, P450 Red, P450 reductase, P450R, P450REd, POR, RedA, reduced nicotinamide adenine dinucleotide phosphate-cytochrome c reductase, reductase, cytochrome c (reduced nicotinamide adenine dinucleotide phosphate), SbCPR2, TcCPR-A, TcCPR-B, TcCPR-C, TPNH-cytochrome c reductase, TPNH2 cytochrome c reductase, WsCPR1, WsCPR2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.2 With a heme protein as acceptor
                1.6.2.4 NADPH-hemoprotein reductase

Specific Activity

Specific Activity on EC 1.6.2.4 - NADPH-hemoprotein reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.002
-
outer membrane of mitochondria
0.00271
-
microsomes
0.0085
-
whole homogenate, prepared from 6 day-organisms, centrifugation for 165 min
0.0095
-
post-mitochondrial supernatant, prepared from 6 day-organisms, centrifugation for 165 min
0.0097
-
post-mitochondrial supernatant, prepared from 1 day-organisms, centrifugation for 6 h
0.0104
-
aortic microsomal preparation
0.01087
-
after cell breakage with glass beads and for 30 min
0.0116
-
whole homogenate, prepared from 1 day-organisms, centrifugation for 165 min
0.0128
-
endogenous enzyme
0.0133
-
fraction precipitated with 40-50% ammonium sulfate, 3-methylcholantrene treated-animals
0.0135
-
whole homogenate, prepared from 1 day-organisms, centrifugation for 6 h
0.0144
-
post-mitochondrial supernatant, prepared from 1 day-organisms, centrifugation for 165 min
0.0173
-
endogenous enzyme
0.0196
-
mitochondria
0.02 - 0.12
-
flower buds, open flowers exhibits a similar value
0.0204
-
inner membrane plus matrix
0.021
-
riboflavin-deficient animals, feeding period 7 weeks, electron acceptor: cytochrome c
0.0223
-
spheroplasts
0.0224
-
inner membrane
0.0233
-
-
0.025
-
electron donor: NADH, electron acceptor: dichlorophenolindophenol
0.029
-
malpighian tubules
0.03
-
electron donor: NADPH, electron acceptor: cytochrome b5
0.033
-
endoplasmic reticulum
0.034
-
beta-naphthoflavone-treated animals
0.036
0.04
-
microsomes, 3-methylcholantrene treated-animals
0.0438
-
after cell breakage through filter screen and centrifugation for 30 min
0.048
-
microsomes, control animals
0.05
-
cholate and Lubrol Px
0.055
-
electron donor: NADH, electron acceptor: cytochrome c
0.059
-
no detergent
0.06572
-
purified microsomal ethanol-oxidizing system fraction
0.066
-
riboflavin-deficient animals, feeding period 6 weeks, electron acceptor: cytochrome c
0.075
-
riboflavin deficient animals, feeding period 7 weeks, electron acceptor: ferricyanide
0.0775
-
purified enzyme
0.079
-
females
0.0857
-
outer membrane
0.08572
-
microsomes
0.087
-
microsomes
0.097
-
riboflavin-deficient-animals, feeding period 6 weeks, electron acceptor: ferricyanide
0.1 - 0.3
-
grown on glycerol, microsomal fraction
0.105
-
microsomes, phenobarbital-treated animals
0.119
-
105000 x g pellet, microsomes
0.124 - 0.138
-
fraction precipitated with 40-50% ammonium sulfate, 3-methylcholantrene-treated animals
0.144
-
fraction precipitated with 40-50% ammonium sulfate, control animals
0.157
-
control animals, feeding period 6 weeks, electron acceptor: ferricyanide
0.165
-
control animals, feeding period 6 weeks, electron acceptor: cytochrome c
0.17
R454E mutant, electron acceptor: cytochrome c
0.171
-
nuclear envelope
0.189
-
electron acceptor: formylated cytochrome c
0.211
-
brain, electron acceptor: hexadecanal
0.225 - 0.38
-
endogenous enzyme
0.23
Y456S mutant, electron acceptor: cytochrome c
0.245
-
purification by ion exchange chromatography and gel filtration, electron acceptor: dichlorophenolindophenol
0.258 - 0.276
-
fraction precipitated with 40-50% ammonium sulfate, phenobarbital-treated animals
0.28
-
Y140D/178D double mutant, electron acceptor: cytochrome c
0.3 - 0.7
-
-
0.333
-
microsomes
0.361
-
coexpression of 17alpha-2D6 + pJR7, supplementation of cultures with delta-aminolevulinic acid
0.414
-
coexpression of 17alpha-2D6 + pJR7, without supplementation of cultures with delta-aminolevulinic acid
0.44
-
Y178D mutant, electron acceptor: cytochrome c
0.517
-
coexpression of ompA-2D6 + pJR7, supplementation of cultures with delta-aminolevulinic acid
0.58
-
peroxidation of microsomes for 18 min
0.601
-
coexpression of ompA-2D6 + pJR7, without supplementation of cultures with delta-aminolevulinic acid
0.676
-
purified enzyme, liver
0.7
-
under carbon limitation
0.72
-
control microsomes
0.75 - 1.1
-
grown on alkane, microsomal fraction
0.788
-
mitochondria
0.928
-
electron acceptor: cytochrome c
1
-
under oxygen limitation
1.12
-
purification by ion exchange chromatography and gel filtration, electron acceptor: ferricyanide
1.2
-
soybean oil or malt extract as carbon source, high aeration
1.35
G488L mutant, electron acceptor: cytochrome c
1.429
-
purification by ion exchange chromatography and gel filtration
1.696
-
recombinant enzyme coexpressed with CYP3A4 in Spodoptera frugiperda cell lines
1.7
W677X mutant, electron acceptor: cytochrome c
10.5
-
glycerol as carbon source, low aeration
11
-
Y140D mutant, electron acceptor: cytochrome c
113
-
Y178F mutant, electron acceptor: ferricyanide
12.5
-
grown on alkane, electron acceptor: neotetrazolium chloride, purified enzyme
13.1
-
endoplasmic reticulum
14.5
-
purified enzyme with cytochrome c as substrate, pH 7.7
14.76
-
purified truncated enzyme, pH 7.8, 25°C
15.2
-
polymorphonuclear leukocytes
16.5
-
cDNA-expressed liver enzyme
17.1
-
liver
17.14
-
purification by affinity chromatography on 2',5'-ADP-Sepharose 4B
17.9
-
electron donor: NADPH, electron acceptor: dichlorophenolindophenol
18.1
-
mitochondria
18.4
-
electron acceptor: 2,6-dichlorophenolindophenol
18.5
-
grown on alkane, electron acceptor: ferricyanide, purified enzyme
19
-
electron acceptor: neotetrazolium chloride
2.15
isozyme CPR2, using ferricyanide as cosubstrate, at 25°C, pH 7.4
2.6
-
glucose as carbon source, low aeration
22
-
in the presence of 0.005 mM FMN
24.3
-
purified truncated protein
26.1
-
-
28
-
electron acceptor: menadione
29
-
grown on alkane, electron acceptor: menadione, purified enzyme
3.17
isozyme CPR2, using ferricytochrome c as cosubstrate, at 25°C, pH 7.4
3.27
-
purified full length enzyme, pH 7.8, 25°C
3.3
isozyme CPR2, for NADPH with cytochrome c as cosubstrate, at 25°C, pH 7.4
3.47
isozyme CPR1, using ferricyanide as cosubstrate, at 25°C, pH 7.4
3.904
-
recombinant enzyme coexpressed with CYP3A4 in Trichoplusia ni cell lines
32.7
T491V mutant, electron acceptor: cytochrome c
37.6
-
purified enzyme
4.69
isozyme CPR1, using ferricytochrome c as cosubstrate, at 25°C, pH 7.4
4.78
isozyme CPR1, for NADPH with cytochrome c as cosubstrate, at 25°C, pH 7.4
40.7
-
Golgi apparatus
41.1
-
plasma membrane
41.2
-
electron donor: NADPH, electron acceptor: cytochrome c
42
-
electron acceptor: dichlorophenolindophenol
46
-
Y140F/178F double mutant, electron acceptor: cytochrome c
47.4
-
Y178F mutant, electron acceptor: cytochrome c
48.1
-
Y178D mutant, electron acceptor: ferricyanide
5.5
-
preparative polyacrylamide disc gel electrophoresis
5.7
-
soybean oil as carbon source, low aeration
5.8
at pH 7.8 and 25°C
5.9
-
brain, electron acceptor: p-nitrobenzaldehyde
50
-
grown on alkane, electron acceptor: dichlorophenolindophenol, purified enzyme
51
-
Y140D/178D double mutant, electron acceptor: ferricyanide
51.5
-
bacterially expressed reductase protein, electron acceptor: cytochrome c
52.9
-
electron acceptor: ferricyanide
53.3
-
electron acceptor: cytochrome c
55.2
-
Y140F mutant, electron acceptor: cytochrome c
57.4
wild type, electron acceptor: cytochrome c
6.83
purified recombinant enzyme, pH 7.8, 25°C
60
-
purified enzyme, microsomes
60 - 120
-
purified enzyme
63.8
-
purification by n-octylamino-Sepharose 4B and 2',5'-ADP column chromatography
65.4
C472T mutant, electron acceptor: cytochrome c
7.56
purified recombinant enzyme, pH 7.8, 25°C
70
-
grown on alkane, electron acceptor: cytochrome c, purified enzyme
8.64
-
electron acceptor: dichlorophenolindophenol
9.32
isozyme CPR1, using dichlorophenolindophenol as cosubstrate, at 25°C, pH 7.4
9.84
isozyme CPR2, using dichlorophenolindophenol as cosubstrate, at 25°C, pH 7.4
94.2
-
Y140F/178F double mutant, electron acceptor: ferricyanide
94.8
-
Y140F mutant, electron acceptor: ferricyanide
98.3
-
electron acceptor: ferricyanide
additional information