1.6.5.4: monodehydroascorbate reductase (NADH)

This is an abbreviated version!
For detailed information about monodehydroascorbate reductase (NADH), go to the full flat file.

Word Map on EC 1.6.5.4

Reaction

NADH
+
H+
+ 2 monodehydroascorbate =
NAD+
+ 2 ascorbate

Synonyms

AFR reductase, AFR-reductase, ascorbate free radical reductase, ascorbate free-radical reductase, ascorbic free radical reductase, At3g09940, AtMDAR1, defensin, MDA, MDA reductase, MDAR, MDAR-OX, MDAR2, MDAR5, MDAsA reductase (NADPH), MDHA, MDHAR, MDHAR1, MDHAR2, MDHAR3, MDHAR6, metallothionein-like type 1 protein, monodehydroascorbate radical reductase, monodehydroascorbate reductase, monodehydroascorbate reductase 2, monodehydroascorbate reductase 4, More, MT-2, MT1K, NADH-semidehydroascorbate oxidoreductase, NADH:AFR oxidoreductase, NADH:ascorbate radical oxidoreductase, NADH:semidehydroascorbic acid oxidoreductase, NEC3, Nectarin III, Os09g0567300, peroxisomal monodehydroascorbate reductase, PpMDHAR1, PpMDHAR2, PpMDHAR3, SDA reductase, semidehydroascorbate reductase, SOR, SPD1, thioredoxin H2, Trx h2

ECTree

     1 Oxidoreductases
         1.6 Acting on NADH or NADPH
             1.6.5 With a quinone or similar compound as acceptor
                1.6.5.4 monodehydroascorbate reductase (NADH)

Purification

Purification on EC 1.6.5.4 - monodehydroascorbate reductase (NADH)

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
Butyl-Toyopearl column chromatography, HiTrap Q column chromatography and Blue Sepharose 6FF column chromatography
-
glutathione Sepharose column chromatography
-
Ni-NTA agarose chromatography
Phenyl Superose gel filtration, Blue Sepharose column chromatography and DEAE cellulose column chromatography
-
recombinant enzyme
recombinant from Escherichia coli
-
several native enzyme forms from lens soluble fraction by gel filtration, affinity chromatography, native isoelectric focusing, and 2D-electrophoresis, about 1400fold
-