1.6.99.1: NADPH dehydrogenase

This is an abbreviated version!
For detailed information about NADPH dehydrogenase, go to the full flat file.

Reaction

Synonyms

2-cyclohexen-1-one, dehydrogenase, reduced nicotinamide adenine dinucleotide phosphate, diaphorase, dihydronicotinamide adenine dinucleotide phosphate dehydrogenase, EBP, Estrogen-binding protein, NADP-d, NADPH diaphorase, NADPH-d, NADPH-dehydrogenase, NADPH-dependent cyclohexenone reductase, NADPH-dependent diaphorase, NADPH-diaphorase, NADPH-methemoglobin reductase, NADPH2-dehydrogenase, NCR, nicotinamide adenine dinucleotide phosphate diaphorase, nitric oxide synthase, old yellow enzyme, Old yellow enzyme homolog, OYE, OYE1, OYE2, OYE3, reduced nicotinamide adenine dinucleotide phosphate dehydrogenase, SYE4, TPNH dehydrogenase, TPNH-diaphorase, triphosphopyridine diaphorase, triphosphopyridine nucleotide diaphorase, YqjM

ECTree

     1 Oxidoreductases

         1.6 Acting on NADH or NADPH

             1.6.99 With unknown physiological acceptors

                1.6.99.1 NADPH dehydrogenase

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Results	in table
3083	AA Sequence
3	Application
1	CAS Registry Number
7	Cloned(Commentary)
34	Cofactor
4	Crystallization (Commentary)
13818	Disease/ Diagnostics
1	Expression
4	General Information
10	Inhibitors
1	Ki Value [mM]
23	KM Value [mM]
4	Localization
2	Metals/Ions
17	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
316	Organism
87	PDB ID
2	pH Optimum
2	Protein Variants
6	Purification (Commentary)
3	Reaction
3	Reaction Type
45	Reference
36	Source Tissue
66	Substrates and Products (Substrate)
11	Subunits
53	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
23	Turnover Number [1/s]

Engineering

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Engineering on EC 1.6.99.1 - NADPH dehydrogenase

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

Y196F

Saccharomyces pastorianus

-

slowing by nearly 6 orders of magnitude of its oxidative half-reaction with 2-cyclohexenone

392801

additional information

Saccharomyces cerevisiae

-

enzyme deletion strain, shows overly elaborate actin cytoskeleton probably reflecting stabilization of actin filaments, and is sensitive to oxidative stress. Phenotype is suppressed by mutation of either conserved cysteine of the pair C285-C374 of actin

656762