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1.7.1.1: nitrate reductase (NADH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADH), go to the full flat file.

Word Map on EC 1.7.1.1

Reaction

nitrite
+
NAD+
+
H2O
=
nitrate
+
NADH
+
H+

Synonyms

assimilatory NADH:nitrate reductase, Assimilatory nitrate reductase, cytosolic NADH nitrate reductase, EC 1.6.6.1, EC 1.7.99.4, MSMEG_4206, MSMEI_4108, NADH-dependent nitrate reductase, NADH-Nar, NADH-nitrate reductase, NADH-NO3- reductase, NADH:nitrate oxidoreductase, NaR, NaR1, NasA, NasC, NIA1, Nia2, nitrate reductase, NR, NR1, NR2, Pden_4449, reductase, nitrate

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.1 nitrate reductase (NADH)

Cloned

Cloned on EC 1.7.1.1 - nitrate reductase (NADH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Nicotiana plumbaginifolia
-
expression in Pichia pastoris
-
expression of amino-terminal, molybdenum-containing domain in Escherichia coli either as His-tagged or GST-tagged fusion protein
-
gene nar1, genotyping of wild-type and mutant nar1 enzyme, overview
-
gene nasA, DNA and amino acid sequence determination and analysis, sequence comparisons and detailed phylogenetic analysis, overview
gene niaD, DNA and amino acid sequence determination and analysis, functional complemention of the defective NO3- assimilation by mutant strain M10, expression in strain T1
high-level expression of the catalytically active flavin domain in Escherichia coli
mutant S534A is expressed in Pichia pastoris strainKM71
-
recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia1-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 628-917
recombinant expression as two separate fragments in Escherichia coli, the N-terminal Nia2-Mo-heme fragment and a C-terminal fragment containing hinge 2 and the FAD-domain, residues 626-917
the C-terminal 268 residues corresponding to the flavin-containing domain, amplified and expressed in Escherichia coli
-
two homologous genes coding for nitrate reductase in tobacco
-