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1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

anthranilate
+
N,N-dimethyl-1,4-phenylenediamine
+ 2 NAD+ =
2-(4-dimethylaminophenyl)diazenylbenzoate
+ 2 NADH + 2 H+

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.17 FMN-dependent NADH-azoreductase

General Stability

General Stability on EC 1.7.1.17 - FMN-dependent NADH-azoreductase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
50% of azoreductase is unfolded up to 2 M urea and complete denaturation stage is up to 4 M urea concentration, respectively. 55% of azoreductase is unfolded up to 2 M GdmCl condition
activity is oxygen insensitive
more than 90% decolorization efficiency is observed under high-salt conditions (5-40 g/l NaCl)
-
non-immobilized enzyme shows a constant decrease in activity to become entirely inactive after 35 days, unlike activities of the immobilized biocatalysts (meso-cellular foams and mesoporous silica) which decline significantly during the first week and then they stabilize to retain ca. 27%-42% of the initial enzyme activity after 63 days
-
the recombinant enzyme is an extremely stable indigo reductase