1.7.1.17: FMN-dependent NADH-azoreductase
This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.
Reaction
+
+
2 NAD+
=
+
2 NADH
+
2 H+
Synonyms
acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase
ECTree
KCat KM Value
KCat KM Value on EC 1.7.1.17 - FMN-dependent NADH-azoreductase
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0.03
Methyl red
mutant enzyme N96A, at pH 7.4 and 30°C
0.09
Methyl red
mutant enzyme H143A, at pH 7.4 and 30°C
0.13
Methyl red
mutant enzyme S16A, at pH 7.4 and 30°C
0.45
Methyl red
mutant enzyme R139A, at pH 7.4 and 30°C
0.8
Methyl red
mutant enzyme R59A, at pH 7.4 and 30°C
1.04
Methyl red
mutant enzyme Y119A, at pH 7.4 and 30°C
2.93
Methyl red
wild type enzyme, at pH 7.4 and 30°C
4.39
Methyl red
mutant enzyme F117A, at pH 7.4 and 30°C
0.03
NADH
mutant enzyme Y119A, at pH 7.4 and 30°C
0.04
NADH
mutant enzyme S16A, at pH 7.4 and 30°C
0.05
NADH
mutant enzyme N96A, at pH 7.4 and 30°C
0.07
NADH
mutant enzyme H143A, at pH 7.4 and 30°C
0.09
NADH
mutant enzyme R59A, at pH 7.4 and 30°C
0.14
NADH
mutant enzyme R139A, at pH 7.4 and 30°C
0.18
NADH
wild type enzyme, at pH 7.4 and 30°C
2.51
NADH
mutant enzyme F117A, at pH 7.4 and 30°C