1.7.1.17: FMN-dependent NADH-azoreductase
This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.
Reaction
Synonyms
acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase
ECTree
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KM Value
KM Value on EC 1.7.1.17 - FMN-dependent NADH-azoreductase
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0.0179
2-(4-dimethylaminophenyl)diazenylbenzoate
recombinant enzyme, pH 7.4, 30°C
0.061
wild type enzyme, at pH 8.5 and 25°C
0.062
1,2-naphthoquinone
mutant enzyme W60A, at pH 8.5 and 25°C
0.064
mutant enzyme W60A, at pH 8.5 and 25°C
0.097
1,4-Naphthoquinone
wild type enzyme, at pH 8.5 and 25°C
0.002
cofactor roseoflavin mononucleotide, pH 7.4, 30°C
0.011
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant R21G/N121A, pH 7.1, temperature not specified in the publication
0.016
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant R21G/N121Q, pH 7.1, temperature not specified in the publication
0.02
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant R21K, pH 7.1, temperature not specified in the publication
0.024
4'-(dimethylamino)-azobenzene-2-carboxylic acid
pH 7.1, 23°C
0.024
4'-(dimethylamino)-azobenzene-2-carboxylic acid
wild-type, pH 7.1, temperature not specified in the publication
0.0247
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant W105F, pH 7.1, 23°C
0.0251
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant W105H, pH 7.1, 23°C
0.0254
4'-(dimethylamino)-azobenzene-2-carboxylic acid
wild-type, pH 7.1, 23°C
0.027
4'-(dimethylamino)-azobenzene-2-carboxylic acid
cofactor FMN, pH 7.4, 30°C
0.0276
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant W105Y, pH 7.1, 23°C
0.0282
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant W105Q, pH 7.1, 23°C
0.029
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant N121Q, pH 7.1, temperature not specified in the publication
0.031
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant R21K/N121A, pH 7.1, temperature not specified in the publication
0.034
4'-(dimethylamino)-azobenzene-2-carboxylic acid
mutant R21K/N121Q, pH 7.1, temperature not specified in the publication
0.025
mutant enzyme W60A, at pH 8.5 and 25°C
0.08
NADH
mutant R21G/N121A, pH 7.1, temperature not specified in the publication
0.115
NADH
mutant R21K/N121Q, pH 7.1, temperature not specified in the publication
0.122
NADH
mutant R21K, pH 7.1, temperature not specified in the publication
0.143
NADH
wild-type, pH 7.1, temperature not specified in the publication
0.19
NADH
mutant R21G/N121Q, pH 7.1, temperature not specified in the publication
0.267
NADH
mutant R21K/N121Q, pH 7.1, temperature not specified in the publication
0.371
NADH
mutant N121Q, pH 7.1, temperature not specified in the publication
0.157
Orange I
mutant enzyme W60A, at pH 8.5 and 25°C
additional information
Michaelis-Menten kinetics
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additional information
additional information
steady-state kinetic analysis
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additional information
additional information
nonlinear Michaelis-Menten kinetics
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