1.7.1.17: FMN-dependent NADH-azoreductase
This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.
Reaction
Synonyms
acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase
ECTree
1.7.1.17 FMN-dependent NADH-azoreductaseAdvanced search results
results (
results (Results
in table
22379
61
49
21
69
Purification
Purification on EC 1.7.1.17 - FMN-dependent NADH-azoreductase
Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
top
PURIFICATION (Commentary) 
ORGANISM
UNIPROT
LITERATURE
native enzyme 4500fold by anion exchange chromatography, Blue Sepharose affinity chromtography, gel filtration, hydroxyapatite chromatography, again anion exchange chromatography and hydrophobic interaction chromatography. Recombinant enzyme AcpD from Escherichia coli, strain JM109 by anion exchange chromatography, Blue Sepharose affinity chromtography, gel filtration, and chromatography on GIGAPITE resin, the purified gene product exhibits activity corresponding to that of the native azoreductase
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged enzyme from Escherichia coli strain XL1-Blue by nickel affinity chromatography
recombinant His-tagged wild-type and mutant AzoR enzymes from Escherichia coli BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzyme from Escherichia coli strain BL21(DE3) by cobalt affinity chromatography, dialysis, and ultrafiltration
