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1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

anthranilate
+
N,N-dimethyl-1,4-phenylenediamine
+ 2 NAD+ =
2-(4-dimethylaminophenyl)diazenylbenzoate
+ 2 NADH + 2 H+

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.17 FMN-dependent NADH-azoreductase

Reference

Reference on EC 1.7.1.17 - FMN-dependent NADH-azoreductase

Please use the Reference Search for a specific query.
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ito, K.; Nakanishi, M.; Lee, W.-C.; Sasaki, H.; Zenno,S.; Saigo, K.; Kitade, Y.; Tanokura, M.
Crystallization and preliminary X-ray analysis of AzoR (azoreductase) from Escherichia coli
Acta Crystallogr. Sect. F
61
399-402
2005
Escherichia coli (P41407)
-
Manually annotated by BRENDA team
Nakanishi, M.; Yatome, C.; Ishida, N.; Kitade, Y.
Putative ACP phosphodiesterase gene (acpD) encodes an azoreductase
J. Biol. Chem.
276
46394-46399
2001
Escherichia coli (P41407)
-
Manually annotated by BRENDA team
Ito, K.; Nakanishi, M.; Lee, W.-C.; Zhi, Y.; Sasaki, H.; Zenno, S.; Saigo, K.; Kitade, Y.; Tanokura, M.
Expansion of substrate specificity and catalytic mechanism of azoreductase by X-ray crystallography and site-directed mutagenesis
J. Biol. Chem.
283
13889-13896
2008
Escherichia coli (P41407)
-
Manually annotated by BRENDA team
Qi, J.; Schloemann, M.; Tischler, D.
Biochemical characterization of an azoreductase from Rhodococcus opacus 1CP possessing methyl red degradation ability
J. Mol. Catal. B
130
9-17
2016
Rhodococcus opacus (A0A1B1KJ01), Rhodococcus opacus 1CP (A0A1B1KJ01)
-
Manually annotated by BRENDA team
Mercier, C.; Chalansonnet, V.; Orenga, S.; Gilbert, C.
Characteristics of major Escherichia coli reductases involved in aerobic nitro and azo reduction
J.Appl. Microbiol.
115
1012-1022
2013
Escherichia coli (P41407)
-
Manually annotated by BRENDA team
Correia, B.; Chen, Z.; Mendes, S.; Martins, L.; Bento, I.
Crystallization and preliminary X-ray diffraction analysis of the azoreductase PpAzoR from Pseudomonas putida MET94
Acta Crystallogr. Sect. F
67
121-123
2011
Pseudomonas putida (Q88IY3), Pseudomonas putida DSM 6125 (Q88IY3)
Manually annotated by BRENDA team
Ooi, T.; Shibata, T.; Sato, R.; Ohno, H.; Kinoshita, S.; Thuoc, T.; Taguchi, S.
An azoreductase, aerobic NADH-dependent flavoprotein discovered from Bacillus sp. Functional expression and enzymatic characterization
Appl. Microbiol. Biotechnol.
75
377-386
2007
Bacillus sp. B29 (Q0WXX2)
Manually annotated by BRENDA team
Matsumoto, K.; Mukai, Y.; Ogata, D.; Shozui, F.; Nduko, J.M.; Taguchi, S.; Ooi, T.
Characterization of thermostable FMN-dependent NADH azoreductase from the moderate thermophile Geobacillus stearothermophilus
Appl. Microbiol. Biotechnol.
86
1431-1438
2010
Geobacillus stearothermophilus (Q9X4K2)
Manually annotated by BRENDA team
Liu, Z.; Chen, H.; Shaw, N.; Hopper, S.; Chen, L.; Chen, S.; Cerniglia, C.; Wang, B.
Crystal structure of an aerobic FMN-dependent azoreductase (AzoA) from Enterococcus faecalis
Arch. Biochem. Biophys.
463
68-77
2007
Enterococcus faecalis (Q831B2)
Manually annotated by BRENDA team
Sun, J.; Kweon, O.; Jin, J.; He, G.; Li, X.; Cerniglia, C.; Chen, H.
Mutation network-based understanding of pleiotropic and epistatic mutational behavior of Enterococcus faecalis FMN-dependent azoreductase
Biochem. Biophys. Rep.
12
240-244
2017
Enterococcus faecalis (Q831B2), Enterococcus faecalis ATCC 700802 (Q831B2)
Manually annotated by BRENDA team
Langer, S.; Nakanishi, S.; Mathes, T.; Knaus, T.; Binter, A.; Macheroux, P.; Mase, T.; Miyakawa, T.; Tanokura, M.; Mack, M.
The flavoenzyme azobenzene reductase AzoR from Escherichia coli binds roseoflavin mononucleotide (RoFMN) with high affinity and is less active in its RoFMN form
Biochemistry
52
4288-4295
2013
Escherichia coli (P41407)
Manually annotated by BRENDA team
Misal, S.A.; Lingojwar, D.P.; Lokhande, M.N.; Lokhande, P.D.; Gawai, K.R.
Enzymatic transformation of nitro-aromatic compounds by a flavin-free NADH azoreductase from Lysinibacillus sphaericus
Biotechnol. Lett.
36
127-131
2014
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Feng, J.; Han, T.; Zhang, M.; Zhou, Y.; Wu, Q.
Application of 2D fluorescence correlation method to investigate the dilution-induced heterogeneous distribution of the bound FMN in azoreductase
Chin. Chem. Lett.
26
210-214
2015
Escherichia coli
-
Manually annotated by BRENDA team
Punj, S.; John, G.
Purification and identification of an FMN-dependent NAD(P)H azoreductase from Enterococcus faecalis
Curr. Issues Mol. Biol.
11
59-66
2009
Enterococcus faecalis, Enterococcus faecalis ATCC 27274
Manually annotated by BRENDA team
Ooi, T.; Ogata, D.; Matsumoto, K.; Nakamura, G.; Yu, J.; Yao, M.; Kitamura, M.; Taguchi, S.
Flavin-binding of azoreductase Direct evidences for dual-binding property of apo-azoreductase with FMN and FAD
J. Mol. Catal. B
74
204-208
2012
Bacillus sp. (in: Bacteria) (Q0WXX2), Bacillus sp. (in: Bacteria) B29 (Q0WXX2)
-
Manually annotated by BRENDA team
Chen, H.; Xu, H.; Kweon, O.; Chen, S.; Cerniglia, C.
Functional role of Trp-105 of Enterococcus faecalis azoreductase (AzoA) as resolved by structural and mutational analysis
Microbiology
154
2659-2667
2008
Enterococcus faecalis (Q831B2), Enterococcus faecalis ATCC 700802 (Q831B2)
Manually annotated by BRENDA team
Chen, H.; Wang, R.; Cerniglia, C.
Molecular cloning, overexpression, purification, and characterization of an aerobic FMN-dependent azoreductase from Enterococcus faecalis
Protein Expr. Purif.
34
302-310
2004
Enterococcus faecalis (Q831B2), Enterococcus faecalis ATCC 19433 (Q831B2)
Manually annotated by BRENDA team
Romero, E.; Savino, S.; Fraaije, M.W.; Loncar, N.
Mechanistic and crystallographic studies of azoreductase AzoA from Bacillus wakoensis A01
ACS Chem. Biol.
15
504-512
2020
Alkalihalobacillus wakoensis (A0A455X8R2), Alkalihalobacillus wakoensis A01 (A0A455X8R2)
Manually annotated by BRENDA team
Valenzuela-Garcia, L.I.; Zapata, B.L.; Ramirez-Ramirez, N.; Huchin-Mian, J.P.; Robleto, E.A.; Ayala-Garcia, V.M.; Pedraza-Reyes, M.
Novel biochemical properties and physiological role of the flavin mononucleotide oxidoreductase YhdA from Bacillus subtilis
Appl. Environ. Microbiol.
86
e01688-20
2020
Bacillus smithii (A0A0H4NXC5), Bacillus smithii DSM 4216 (A0A0H4NXC5)
Manually annotated by BRENDA team
Verma, K.; Kundu, D.; Kundu, L.M.; Singh, A.K.; Dubey, V.K.
Folding and stability of recombinant azoreductase enzyme from Chromobacterium violaceum
Enzyme Microb. Technol.
131
109433
2019
Chromobacterium violaceum (Q7NWH3), Chromobacterium violaceum ATCC 12472 (Q7NWH3)
Manually annotated by BRENDA team
Qi, J.; Anke, M.; Szymanska, K.; Tischler, D.
Immobilization of Rhodococcus opacus 1CP azoreductase to obtain azo dye degrading biocatalysts operative at acidic pH
Int. Biodeter. Biodegrad.
118
89-94
2017
Rhodococcus opacus, Rhodococcus opacus 1CP
-
Manually annotated by BRENDA team
Cao, X.; Di, M.; Wang, J.
Expansion of the active site of the azoreductase from Shewanella oneidensis MR-1
J. Mol. Graph. Model.
78
213-220
2017
Shewanella oneidensis (Q8E990)
Manually annotated by BRENDA team
Zahran, S.; Ali-Tammam, M.; Hashem, A.; Aziz, R.; Ali, A.
Azoreductase activity of dye-decolorizing bacteria isolated from the human gut microbiota
Sci. Rep.
9
5508
2019
Bacillus cereus, Escherichia coli, Enterococcus faecalis, Enterococcus avium
Manually annotated by BRENDA team
Fazeliyan, E.; Sadeghi, M.; Forouzandeh, S.; Doosti, A.; Mohammadi Moghadam, F.; Sedehi, M.; Emadi, Z.; Sadeghi, R.
Decolorization mechanism, identification of an FMN-dependent NADH-azoreductase from a moderately halotolerant Staphylococcus sp. MEH038S, and toxicity assessment of biotransformed metabolites
Water Environ. Res.
93
2072-2083
2021
Staphylococcus sp. MEH038S
Manually annotated by BRENDA team