1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

+ 2 NAD⁺ =

2-(4-dimethylaminophenyl)diazenylbenzoate

+ 2 NADH + 2 H+

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

1 Oxidoreductases

1.7 Acting on other nitrogenous compounds as donors

1.7.1 With NAD⁺ or NADP⁺ as acceptor

1.7.1.17 FMN-dependent NADH-azoreductase

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Results	in table
22379	AA Sequence
1	Application
16	Cloned(Commentary)
61	Cofactor
7	Crystallization (Commentary)
10	General Information
5	General Stability
10	Inhibitors
16	kcat/KM [mM/s]
67	KM Value [mM]
5	Localization
2	Metals/Ions
8	Molecular Weight [Da]
18	Natural Substrates/ Products (Substrates)
49	Organism
1	Oxidation Stability
7	PDB ID
7	pH Optimum
2	pH Stability
1	pI Value
43	Protein Variants
10	Purification (Commentary)
5	Reaction
38	Reference
2	Source Tissue
22	Specific Activity [micromol/min/mg]
106	Substrates and Products (Substrate)
21	Subunits
69	Synonyms
1	Systematic Name
9	Temperature Optimum [°C]
1	Temperature Range [°C]
10	Temperature Stability [°C]
24	Turnover Number [1/s]

Systematic Name

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Systematic Name on EC 1.7.1.17 - FMN-dependent NADH-azoreductase

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N,N-dimethyl-1,4-phenylenediamine, anthranilate:NAD+ oxidoreductase

Requires FMN. The enzyme catalyses the reductive cleavage of an azo bond in aromatic azo compounds to form the corresponding amines. Does not accept NADPH. cf. EC 1.7.1.6, azobenzene reductase.