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1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

anthranilate
+
N,N-dimethyl-1,4-phenylenediamine
+ 2 NAD+ =
2-(4-dimethylaminophenyl)diazenylbenzoate
+ 2 NADH + 2 H+

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.17 FMN-dependent NADH-azoreductase

Temperature Stability

Temperature Stability on EC 1.7.1.17 - FMN-dependent NADH-azoreductase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
10 - 40
purified recombinant enzyme, pH 7.0, 0.1 M sodium phosphate buffer, 30 min, stable at
20 - 100
purified recombinant wild-type enzyme, completely stable, while the mutant shows loss of 50% activity at about 55°C and inactivation at 90°C
45
stable for at least 1 h
55
purified recombinant enzyme, unfolding temperature
70
-
stable for 30 min
71
apparent melting temperature
additional information
the recombinant enzyme is an extremely stable indigo reductase, analysis of the structure of this highly thermostable indigo reductase and identification of the key amino acid residues responsible for the thermostability