1.7.1.17: FMN-dependent NADH-azoreductase

This is an abbreviated version!
For detailed information about FMN-dependent NADH-azoreductase, go to the full flat file.

Reaction

Synonyms

acpD, AZO, azo-dye reductase, AzoA, AzoR, AzoR1, azoreductase, AzoRo, AzrA, AzrG, flavin-containing oxygen-insensitive azoreductase, FMN dependent azoreductase, FMN-dependent methyl red reductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azo reductase, FMN-dependent NADH-azoreductase, FMN-dependent-NADH azoreductase, indigo reductase, NADH-azoreductase

ECTree

     1 Oxidoreductases

         1.7 Acting on other nitrogenous compounds as donors

             1.7.1 With NAD⁺ or NADP⁺ as acceptor

                1.7.1.17 FMN-dependent NADH-azoreductase

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Results

in table

22379

AA Sequence

1

Application

16

Cloned(Commentary)

61

Cofactor

7

Crystallization (Commentary)

10

General Information

5

General Stability

10

Inhibitors

16

kcat/KM [mM/s]

67

KM Value [mM]

5

Localization

2

Metals/Ions

8

Molecular Weight [Da]

18

Natural Substrates/ Products (Substrates)

49

Organism

1

Oxidation Stability

7

PDB ID

7

pH Optimum

2

pH Stability

1

pI Value

43

Protein Variants

10

Purification (Commentary)

5

Reaction

38

Reference

2

Source Tissue

22

Specific Activity [micromol/min/mg]

106

Substrates and Products (Substrate)

21

Subunits

69

Synonyms

1

Systematic Name

9

Temperature Optimum [°C]

1

Temperature Range [°C]

10

Temperature Stability [°C]

24

Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

67 - 111

1,2-naphthoquinone

2 entries

152 - 340

1,4-Naphthoquinone

2 entries

136 - 387

menadione

2 entries

2900 - 70360

Methyl red

8 entries

14420 - 68230

NADH

8 entries

0.85 - 1.06

Orange I

2 entries

67

1,2-naphthoquinone

Alkalihalobacillus wakoensis

A0A455X8R2

mutant enzyme W60A, at pH 8.5 and 25°C

763801

111

1,2-naphthoquinone

Alkalihalobacillus wakoensis

A0A455X8R2

wild type enzyme, at pH 8.5 and 25°C

763801

152

1,4-Naphthoquinone

Alkalihalobacillus wakoensis

A0A455X8R2

mutant enzyme W60A, at pH 8.5 and 25°C

763801

340

1,4-Naphthoquinone

Alkalihalobacillus wakoensis

A0A455X8R2

wild type enzyme, at pH 8.5 and 25°C

763801

136

menadione

Alkalihalobacillus wakoensis

A0A455X8R2

mutant enzyme W60A, at pH 8.5 and 25°C

763801

387

menadione

Alkalihalobacillus wakoensis

A0A455X8R2

wild type enzyme, at pH 8.5 and 25°C

763801

2900

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme N96A, at pH 7.4 and 30°C

765245

6350

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme H143A, at pH 7.4 and 30°C

765245

9800

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme S16A, at pH 7.4 and 30°C

765245

20020

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme Y119A, at pH 7.4 and 30°C

765245

21480

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme R139A, at pH 7.4 and 30°C

765245

23680

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme R59A, at pH 7.4 and 30°C

765245

33860

Methyl red

Shewanella oneidensis

Q8E990

wild type enzyme, at pH 7.4 and 30°C

765245

70360

Methyl red

Shewanella oneidensis

Q8E990

mutant enzyme F117A, at pH 7.4 and 30°C

765245

14420

NADH

Shewanella oneidensis

Q8E990

mutant enzyme N96A, at pH 7.4 and 30°C

765245

17170

NADH

Shewanella oneidensis

Q8E990

mutant enzyme Y119A, at pH 7.4 and 30°C

765245

19180

NADH

Shewanella oneidensis

Q8E990

mutant enzyme S16A, at pH 7.4 and 30°C

765245

29390

NADH

Shewanella oneidensis

Q8E990

mutant enzyme H143A, at pH 7.4 and 30°C

765245

30200

NADH

Shewanella oneidensis

Q8E990

mutant enzyme R139A, at pH 7.4 and 30°C

765245

32130

NADH

Shewanella oneidensis

Q8E990

mutant enzyme R59A, at pH 7.4 and 30°C

765245

33900

NADH

Shewanella oneidensis

Q8E990

wild type enzyme, at pH 7.4 and 30°C

765245

68230

NADH

Shewanella oneidensis

Q8E990

mutant enzyme F117A, at pH 7.4 and 30°C

765245

0.85

Orange I

Alkalihalobacillus wakoensis

A0A455X8R2

wild type enzyme, at pH 8.5 and 25°C

763801

1.06

Orange I

Alkalihalobacillus wakoensis

A0A455X8R2

mutant enzyme W60A, at pH 8.5 and 25°C

763801