1.7.1.3: nitrate reductase (NADPH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADPH), go to the full flat file.

Reaction

Synonyms

assimilatory NADPH-nitrate reductase, assimilatory NADPH:nitrate reductase, Assimilatory nitrate reductase, assimilatory reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase, EC 1.6.6.3, EC 1.7.99.4, MSMEG_2837, NADPH-dependent nitrate reductase, NADPH-nitrate reductase, NADPH2:nitrate oxidoreductase, NADPH:nitrate reductase, NADPH:NR, NaR1, narB, nit-3, nitrate reductase, nitrate reductase (NADPH), nitrate reductase (reduced nicotinamide adenine dinucleotide phosphate), nitrate reductase [NADPH], NR, triphosphopyridine nucleotide-nitrate reductase

ECTree

     1 Oxidoreductases

         1.7 Acting on other nitrogenous compounds as donors

             1.7.1 With NAD⁺ or NADP⁺ as acceptor

                1.7.1.3 nitrate reductase (NADPH)

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Results	in table
8	Activating Compound
1181	AA Sequence
6	Application
1	CAS Registry Number
9	Cloned(Commentary)
49	Cofactor
1	Crystallization (Commentary)
4	General Information
2	General Stability
41	Inhibitors
4	Ki Value [mM]
26	KM Value [mM]
1	Localization
27	Metals/Ions
23	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
3	Organic Solvent Stability
40	Organism
4	Pathway
18	pH Optimum
2	pH Range
1	pH Stability
20	Protein Variants
15	Purification (Commentary)
5	Reaction
3	Reaction Type
38	Reference
23	Source Tissue
26	Specific Activity [micromol/min/mg]
13	Storage Stability
65	Substrates and Products (Substrate)
22	Subunits
28	Synonyms
1	Systematic Name
13	Temperature Optimum [°C]
1	Temperature Range [°C]
17	Temperature Stability [°C]
10	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.7.1.3 - nitrate reductase (NADPH)

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-10°C, 0.1 M sodium phosphate buffer, pH 7.3, 0.17 M NaCl, 1 mM dithiothreitol, 5 m M EDTA, 0.5 mM PMSF, 1%ethanol, 30% glycerol

Neurospora crassa

-

395978

-10°C, 50 mM sodium phosphate, pH 6.9, 30% glycerol, 0.5 mM EDTA, 0.5 mM dithiothreitol, 0.5 mM phenylmethylsulfonylfluoride, 0.1 mM FAD, stable for at least 6 months

Neurospora crassa

-

395970

-15°C, fraction II, most stable, optimal pH: 7.0, 10-20% loss of activity after a month

Neurospora crassa

-

395975

-15°C, fraction IV, one week, loses about half of its activity

Neurospora crassa

-

395975

-15°C, fraction V, quite unstable, overnight, loses at least half of its activity

Neurospora crassa

-

395975

-20°C, stable for 6 months or longer, 5% loss of activity

Neurospora crassa

-

395981

-80°C, 0.02 mM FAD, several months with only slight loss of the activity

Aspergillus nidulans

-

395971

-80°C, 25 mM Mops, 0.1 mM EDTA, pH 7.2

Neurospora crassa

-

395976

-80°C, extract, 6 months, without loss of activity

Neurospora crassa

-

395977

4°C or -15°C, fraction III, overnight, 50% loss of activity

Neurospora crassa

-

395975

4°C, half life of 3-5 days

Aspergillus nidulans

-

395968

5°C, urea-treated, 0.1 M potassium phosphate buffer, pH 7.3, 5 mM EDTA, 2 mM dithioerythritol, 0.5 mg/ml or more bovine serum albumin, stable for 18 h without loss of activity

Neurospora crassa

-

395980

half life of about 2 h with ammonium sulfate or ammonium chloride as sole N-source

Funaria hygrometrica

-

395969