Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
0.12
1,2-dimethyl-4-(p-carboxyphenylazo)-5-hydroxy-benzene
-
-
0.0013
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol
-
-
0.0137
1-(2'-sulfophenylazo)-2-naphthol
-
-
0.0015
1-(3'-carboxyphenylazo)-2-naphthol
-
-
0.0105
1-(3'-phosphophenylazo)-2-naphthol
-
-
0.0018
1-(3'-sulfophenylazo)-2-naphthol
-
-
0.013
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene
-
-
0.0198
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene
-
-
0.008
1-(4'-carboxyphenylazo)-2-naphthol
-
-
0.0024
1-(4'-carboxyphenylazo)-4-naphthol
-
-
0.0018
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene
-
-
0.0052
1-(4'-phosphophenylazo)-2-naphthol
-
-
0.0148
1-(4'-sulfoaminophenylazo)-2-naphthol
-
-
0.04
1-(4'-sulfophenyl-N-methylhydrazo)-4-naphthol
-
-
0.0132
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene
-
-
0.017
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene
-
-
0.001
1-(4'-sulfophenylazo)-2-naphthol
-
-
0.204
1-(4'-sulfophenylazo)-4-anthranol
-
-
0.0026
1-(4'-sulfophenylazo)-4-naphthol
-
-
0.0014
1-phenylazo-2-hydroxy-6-carboxynaphthalene
-
-
0.0009
1-phenylazo-2-hydroxy-6-sulfonaphthalene
-
-
0.0346 - 0.0873
2-Hydroxy-1,4-naphthoquinone
1.704
2-methyl-1,4-benzoquinone
-
0.0162
2-methyl-1,4-naphthoquinone
-
0.024 - 0.42
2-[4-(dimethylamino)phenylazo]benzoic acid
0.0102 - 0.101
4'-dimethylaminoazobenzene-2-carboxylic acid
0.0146
9,10-dioxo-9,10-dihydroanthracene-2,6-disulfonic acid
-
0.0081
9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
-
0.001
acid Orange 7
-
after induction with Acid Orange 7
0.093 - 0.45
Acid Orange 8
0.004
Acid Red 151
-
after induction with Acid Orange 7
0.003 - 0.009
acid Red 88
0.0161
anthraquinone-2,6-disulfonate
-
0.0133
anthraquinone-2-sulfonate
-
0.0986 - 0.1029
balsalazide
0.059
BHQ-10
pH not specified in the publication, 22°C
0.2792
Congo red
recombinant enzyme, pH 6.5, 22°C
7.26
Cr(VI)
pH 7.5, 30°C, recombinant His10-tagged enzyme
0.005
Direct blue 15
-
pH not specified in the publication, temperature not specified in the publication
0.00018
FAD
-
pH not specified in the publication, temperature not specified in the publication
0.51
flame orange
pH not specified in the publication, 22°C
0.1056 - 0.3
Methyl orange
0.00053 - 1.21
Methyl red
0.04
naphthol blue black
-
pH 7.0, 30°C
0.063
Navitan fast blue S5R
-
pH 7.0, room temperature
0.0097
o-methyl red
pH not specified in the publication, 22°C
0.1006 - 0.867
p-methyl red
0.22 - 1.118
sulfasalazine
additional information
additional information
-
0.0346
2-Hydroxy-1,4-naphthoquinone
-
0.0873
2-Hydroxy-1,4-naphthoquinone
-
0.024
2-[4-(dimethylamino)phenylazo]benzoic acid
-
pH 7.1, 23°C
0.029
2-[4-(dimethylamino)phenylazo]benzoic acid
-
pH 7.0, 25°C
0.057
2-[4-(dimethylamino)phenylazo]benzoic acid
-
in 25 mM potassium phosphate buffer (pH 7.1), 0.25 mM 2-[4-(dimethylamino)phenylazo]benzoic acid and 0.1 mM NADPH
0.28
2-[4-(dimethylamino)phenylazo]benzoic acid
GST-fusion protein, pH 8.0, 40°C
0.42
2-[4-(dimethylamino)phenylazo]benzoic acid
native protein, pH 8.0, 40°C
0.0102
4'-dimethylaminoazobenzene-2-carboxylic acid
mutant Y120A
0.0419
4'-dimethylaminoazobenzene-2-carboxylic acid
wild-type enzyme
0.0849
4'-dimethylaminoazobenzene-2-carboxylic acid
mutant R59A
0.101
4'-dimethylaminoazobenzene-2-carboxylic acid
mutant F162A
0.093
Acid Orange 8
-
after induction with Acid Orange 7
0.003
acid Red 88
-
after induction with Acid Orange 7
0.0986
balsalazide
wild type enzyme, using NADPH as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
0.1029
balsalazide
mutant enzyme Y131F, using NADPH as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
0.055
balsalzide
-
isoform AzoR2, pH 8.0, temperature not specified in the publication
0.34
balsalzide
-
isoform AzoR3, pH 8.0, temperature not specified in the publication
2.447
balsalzide
-
isoform AzoR1, pH 8.0, temperature not specified in the publication
0.1056
Methyl orange
recombinant enzyme, pH 6.5, 22°C
0.3
Methyl orange
pH not specified in the publication, 22°C
0.00053
Methyl red
mutant R66A, pH 7.0, 22°C
0.002
Methyl red
-
cofactor roseoflavin mononucleotide, pH 7.4, 30°C
0.0028
Methyl red
cosubstrate NADPH, pH 6.5, 25°C
0.00476
Methyl red
recombinant chimeric fusion protein AZR-GDH, pH 7.0, 40°C
0.0082
Methyl red
mutant N121A, pH 7.0, 22°C
0.00916
Methyl red
recombinant wild-type AZR, pH 7.0, 40°C
0.0092
Methyl red
cosubstrate NADH, pH 6.5, 25°C
0.011
Methyl red
-
in 50 mM potassium phosphate buffer pH 7.0 at 25°C
0.0112
Methyl red
mutant N106A, pH 7.0, 22°C
0.0126
Methyl red
mutant R21G, pH 7.0, 22°C
0.013
Methyl red
-
wild type enzyme, at pH 7.6 and 25°C
0.0164
Methyl red
pH 7.0, 25°C
0.0167
Methyl red
mutant R18G, pH 7.0, 22°C
0.024
Methyl red
wild-type, pH 7.0, 22°C
0.0247
Methyl red
-
mutant W105F
0.0251
Methyl red
-
mutant W105H
0.0254
Methyl red
-
wild-type enzyme
0.027
Methyl red
-
cofactor FMN, pH 7.4, 30°C
0.0276
Methyl red
-
mutant W105Y
0.0282
Methyl red
-
mutant W105Q
0.0288
Methyl red
mutant L59G, pH 7.0, 22°C
0.0335
Methyl red
mutant W62A, pH 7.0, 22°C
0.044
Methyl red
-
mutant enzyme L49C/D108C, at pH 7.6 and 25°C
0.0445
Methyl red
mutant enzyme Y131F, using NADPH as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
0.0485
Methyl red
mutant A123F, pH 7.0, 22°C
0.05
Methyl red
-
pH 7.4, 37°C
0.05
Methyl red
NADH-dependent, mutant K109H
0.06
Methyl red
NADH-dependent, wild-type enzyme
0.0927
Methyl red
wild type enzyme, using NADPH as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
0.109
Methyl red
mutant V122Y, pH 7.0, 22°C
0.15
Methyl red
NADH-dependent, mutant K109A
0.1593
Methyl red
recombinant enzyme, pH 6.5, 22°C
0.26
Methyl red
-
isoform AzoR1, pH 8.0, temperature not specified in the publication
0.27
Methyl red
wild-type enzyme
0.27
Methyl red
mutant K109H
0.3
Methyl red
-
isoform AzoR2, pH 8.0, temperature not specified in the publication
0.66
Methyl red
mutant K109A
0.8
Methyl red
mutant H75N
0.983
Methyl red
-
isoform AzoR3, pH 8.0, temperature not specified in the publication
1.21
Methyl red
mutant Y74W
0.003
NADH
mutant W62A, pH 7.0, 22°C
0.0081
NADH
-
pH not specified in the publication, temperature not specified in the publication
0.059
NADH
-
pH 7.0, 25°C
0.0706
NADH
wild-type enzyme
0.082
NADH
-
in 50 mM potassium phosphate buffer pH 7.0 at 25°C
0.091
NADH
-
wild type enzyme, at pH 7.6 and 25°C
0.143
NADH
wild-type, pH 7.0, 22°C
0.158
NADH
-
wild-type enzyme
0.166
NADH
-
mutant W105Y
0.169
NADH
-
mutant W105Q
0.171
NADH
-
mutant W105H
0.175
NADH
-
mutant W105F
0.223
NADH
-
mutant enzyme L49C/D108C, at pH 7.6 and 25°C
0.25
NADH
mutant E16G, pH 7.0, 22°C
0.26
NADH
-
cofactor FMN, pH 7.4, 30°C
0.269
NADH
-
cofactor roseoflavin mononucleotide, pH 7.4, 30°C
0.292
NADH
mutant N121A, pH 7.0, 22°C
0.298
NADH
-
isoform AzoR1, pH 8.0, temperature not specified in the publication
0.38
NADH
wild-type enzyme
0.38
NADH
mutant R21G, pH 7.0, 22°C
0.405
NADH
mutant V122Y, pH 7.0, 22°C
0.411
NADH
mutant A123F, pH 7.0, 22°C
0.525
NADH
-
isoform AzoR1, pH 8.0, temperature not specified in the publication
0.538
NADH
wild type enzyme, using methyl red as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
0.581
NADH
mutant R66A, pH 7.0, 22°C
0.624
NADH
mutant R18G, pH 7.0, 22°C
0.793
NADH
mutant N106A, pH 7.0, 22°C
0.965
NADH
mutant enzyme Y131F, using methyl red as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
2.1
NADH
GST-fusion protein, pH 8.0, 40°C
2.298
NADH
mutant L59G, pH 7.0, 22°C
2.3
NADH
native protein, pH 8.0, 40°C
4.35
NADH
-
pH 7.0, room temperature
0.001
NADPH
pH 6.8, 45°C
0.05
NADPH
NADPH-dependent, mutant Y74W
0.06
NADPH
NADPH-dependent, wild-type enzyme
0.074
NADPH
-
in 25 mM potassium phosphate buffer (pH 7.1), 0.25 mM azo dye and 0.1 mM NADPH
0.09
NADPH
wild-type enzyme
0.2
NADPH
NADPH-dependent, mutant K109H
0.37
NADPH
NADPH-dependent, mutant K109A
0.535
NADPH
mutant enzyme Y131F, using methyl red as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
1.197
NADPH
wild type enzyme, using methyl red as cosubstrate, in 20 mM TrisHCl pH 8.0, temperature not specified in the publication
10.26
NADPH
-
isoform AzoR1, pH 8.0, temperature not specified in the publication
15
NADPH
-
in 50 mM potassium phosphate buffer pH 7.0 at 25°C
37.3
NADPH
pH 7.5, 30°C, recombinant His10-tagged enzyme
0.1
nitrofurazone
NADPH-dependent, mutant K109A
0.12
nitrofurazone
NADPH-dependent, mutant K109H
0.13
nitrofurazone
NADPH-dependent, wild-type enzyme
0.65
nitrofurazone
NADPH-dependent, mutant Y74W
0.003
Orange I
using NADPH as proton donor, at pH 6.8, 45°C
0.17
Orange I
using NADH as proton donor, at pH 6.8, 45°C
0.1006
p-methyl red
mutant R59A
0.23
p-methyl red
mutant Y120A
0.266
p-methyl red
wild-type enzyme
0.867
p-methyl red
mutant F162A
0.004
ponceau SX
-
azoreductase I
0.005
ponceau SX
-
azoreductase II
0.22
sulfasalazine
-
isoform AzoR3, pH 8.0, temperature not specified in the publication
0.38
sulfasalazine
-
isoform AzoR2, pH 8.0, temperature not specified in the publication
1.118
sulfasalazine
-
isoform AzoR1, pH 8.0, temperature not specified in the publication
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
-
wild-type and mutant kinetics and thermodynamics, overview
-