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1.7.1.6: azobenzene reductase

This is an abbreviated version!
For detailed information about azobenzene reductase, go to the full flat file.

Word Map on EC 1.7.1.6

Reaction

N,N-dimethyl-1,4-phenylenediamine
+
aniline
+ 2 NADP+ =
4-(dimethylamino)azobenzene
+ 2 NADPH + 2 H+

Synonyms

acpD, aerobic azoreductase, Azo 1, azo dye reductase, azo reductase, azo-dye reductase, azo-reductase, AzoA, AzoB, AzoC, AzoEF1, AzoH, AzoI, AzoII, AzoR, AzoR1, azoreductase, azoreductase 1, azoreductase B, azoreductase C, AZR, AzrA, AzrB, AzrC, AzrG, AzrS, bsAzoR, BsuYhdA, BTI1, class 1 azoreductase, class 2 azoreductase, dibromopropylaminophenylazobenzoic azoreductase, dimethylaminobenzene reductase, EC 1.6.6.7, ecAzoR, EF0404, efAzoR, EF_0404, flavin mononucleotide oxidoreductase, flavin mononucleotide-containing azoreductase, flavin-dependent azoreductase, FMN-dependent NAD(P)H azoreductase, FMN-dependent NAD(P)H nitroreductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azoreductase, methyl red azoreductase, More, N,N-dimethyl-4-phenylazoaniline azoreductase, NAD(P)H:1-(4'-sulfophenylazo)-2-naphthol oxidoreductase, NADH driven FMN dependent azoreductase, NADH-azoreductase, NADPH-dependent azoreductase Azr, NADPH-dependent flavin mononucleotide oxide reductase, NADPH-flavin azoreductase, NADPH2-dependent azoreductase, NC-reductase, new Coccine (NC)-reductase, nicotinamide adenine dinucleotide (phosphate) azoreductase, Orange I azoreductase, Orange II azoreductase, oxygen insensitive intracellular azoreductase, p-aminoazobenzene reductase, p-dimethylaminoazobenzene azoreductase, paAzoR1, PAMD_1, PpAzoR, reductase, azobenzene, rsAzoR, SO 4396, YhdA

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.6 azobenzene reductase

KM Value

KM Value on EC 1.7.1.6 - azobenzene reductase

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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
0.12
1,2-dimethyl-4-(p-carboxyphenylazo)-5-hydroxy-benzene
-
-
0.0013
1-(2'-methyl-4'-sulfophenylazo)-2-naphthol
-
-
0.0137
1-(2'-sulfophenylazo)-2-naphthol
-
-
0.0015
1-(3'-carboxyphenylazo)-2-naphthol
-
-
0.0105
1-(3'-phosphophenylazo)-2-naphthol
-
-
0.0018
1-(3'-sulfophenylazo)-2-naphthol
-
-
0.013
1-(4'-carboxyphenylazo)-2-hydroxy-6-carboxynaphthalene
-
-
0.0198
1-(4'-carboxyphenylazo)-2-hydroxy-6-sulfonaphthalene
-
-
0.008
1-(4'-carboxyphenylazo)-2-naphthol
-
-
0.0024
1-(4'-carboxyphenylazo)-4-naphthol
-
-
0.0018
1-(4'-nitrophenylazo)-2-hydroxy-6-sulfonaphthalene
-
-
0.0052
1-(4'-phosphophenylazo)-2-naphthol
-
-
0.0148
1-(4'-sulfoaminophenylazo)-2-naphthol
-
-
0.04
1-(4'-sulfophenyl-N-methylhydrazo)-4-naphthol
-
-
0.0132
1-(4'-sulfophenylazo)-2-hydroxy-6-carboxynaphthalene
-
-
0.017
1-(4'-sulfophenylazo)-2-hydroxy-6-sulfonaphthalene
-
-
0.001
1-(4'-sulfophenylazo)-2-naphthol
-
-
0.204
1-(4'-sulfophenylazo)-4-anthranol
-
-
0.0026
1-(4'-sulfophenylazo)-4-naphthol
-
-
0.0014
1-phenylazo-2-hydroxy-6-carboxynaphthalene
-
-
0.0009
1-phenylazo-2-hydroxy-6-sulfonaphthalene
-
-
0.0346 - 0.0873
2-Hydroxy-1,4-naphthoquinone
1.704
2-methyl-1,4-benzoquinone
-
0.0162
2-methyl-1,4-naphthoquinone
-
0.024 - 0.42
2-[4-(dimethylamino)phenylazo]benzoic acid
0.0102 - 0.101
4'-dimethylaminoazobenzene-2-carboxylic acid
0.0146
9,10-dioxo-9,10-dihydroanthracene-2,6-disulfonic acid
-
0.0081
9,10-dioxo-9,10-dihydroanthracene-2-sulfonic acid
-
0.001
acid Orange 7
-
after induction with Acid Orange 7
0.093 - 0.45
Acid Orange 8
0.004
Acid Red 151
-
after induction with Acid Orange 7
0.003 - 0.009
acid Red 88
0.034
Amaranth
-
-
0.0161
anthraquinone-2,6-disulfonate
-
0.0133
anthraquinone-2-sulfonate
-
0.0986 - 0.1029
balsalazide
0.055 - 2.447
balsalzide
0.059
BHQ-10
pH not specified in the publication, 22°C
0.00645
CO
-
-
0.2792
Congo red
recombinant enzyme, pH 6.5, 22°C
7.26
Cr(VI)
pH 7.5, 30°C, recombinant His10-tagged enzyme
0.005
Direct blue 15
-
pH not specified in the publication, temperature not specified in the publication
0.00018
FAD
-
pH not specified in the publication, temperature not specified in the publication
0.51
flame orange
pH not specified in the publication, 22°C
0.1056 - 0.3
Methyl orange
0.00053 - 1.21
Methyl red
0.003 - 4.35
NADH
0.001 - 37.3
NADPH
0.04
naphthol blue black
-
pH 7.0, 30°C
0.063
Navitan fast blue S5R
-
pH 7.0, room temperature
0.1 - 0.65
nitrofurazone
0.0097
o-methyl red
pH not specified in the publication, 22°C
0.003 - 0.17
Orange I
0.1006 - 0.867
p-methyl red
0.004 - 0.005
ponceau SX
0.22 - 1.118
sulfasalazine
additional information
additional information
-