1.7.1.6: azobenzene reductase

This is an abbreviated version!
For detailed information about azobenzene reductase, go to the full flat file.

Word Map on EC 1.7.1.6

Reaction

N,N-dimethyl-1,4-phenylenediamine
+
aniline
+ 2 NADP+ =
4-(dimethylamino)azobenzene
+ 2 NADPH + 2 H+

Synonyms

acpD, aerobic azoreductase, Azo 1, azo dye reductase, azo reductase, azo-dye reductase, AzoA, AzoB, AzoC, AzoEF1, AzoI, AzoII, AzoR, AzoR1, azoreductase, azoreductase 1, azoreductase B, azoreductase C, AZR, AzrA, AzrB, AzrC, AzrG, AzrS, bsAzoR, BTI1, class 1 azoreductase, class 2 azoreductase, dibromopropylaminophenylazobenzoic azoreductase, dimethylaminobenzene reductase, EC 1.6.6.7, ecAzoR, EF0404, efAzoR, EF_0404, flavin mononucleotide-containing azoreductase, flavin-dependent azoreductase, FMN-dependent NAD(P)H azoreductase, FMN-dependent NAD(P)H nitroreductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azoreductase, methyl red azoreductase, N,N-dimethyl-4-phenylazoaniline azoreductase, NAD(P)H:1-(4'-sulfophenylazo)-2-naphthol oxidoreductase, NADH driven FMN dependent azoreductase, NADH-azoreductase, NADPH-dependent azoreductase Azr, NADPH-flavin azoreductase, NADPH2-dependent azoreductase, NC-reductase, new Coccine (NC)-reductase, nicotinamide adenine dinucleotide (phosphate) azoreductase, Orange I azoreductase, Orange II azoreductase, oxygen insensitive intracellular azoreductase, p-aminoazobenzene reductase, p-dimethylaminoazobenzene azoreductase, paAzoR1, PAMD_1, PpAzoR, reductase, azobenzene, rsAzoR, SO 4396

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.6 azobenzene reductase

Purification

Purification on EC 1.7.1.6 - azobenzene reductase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
39.3fold by ammonium sulfate fractionation and anion exchange chromatography
-
a Cibacron blue agarose 3G column is used
-
by anion-exchange chromatography and by gel filtration
-
cell extracts of Enterococcus faecalis are prepared, AzoA is purified by hydrophobic, anion exchange and affinity chromatography
-
crude cell extracts are applied on a DEAE cellulose column, subsequently to an ammonium sulfate precipitation the enzyme solution is applied on a Butyl Toyopearl 650M column, finally a Sephacryl S200 column is used; crude cell extracts are applied on a DEAE cellulose column, subsequently to an ammonium sulfate precipitation the enzyme solution is applied on a Phenyl Toyopearl 650M column, finally a Sephacryl S200 column is used
C0STY0
crude protein extracts from Bacillus velezensis are prepared
HiPrep 16/10 Q XL anion-exchange column chromatography and HiLoad 26/60 Superdex 200 gel fltration
-
His-tagged proteins are purified by gravity-flow chromatography using nickel-nitrilotriacetic acid-agarose
-
native intracellular enzyme 94.2fold from mutant strain B1 by ammonium sulfate fracionation and anion exchange chromatography
-
Ni-NTA column chromatography
-
octyl Sepharose column chromatography, DEAE Bio-gel agarose column chromatography, and Affi-gel Blue gel filtration
-
phenyl Sepharose column chromatography and HiPrep SP XL column chromatography
D5HN83
recombinant chimeric fusion protein AZR-GDH from Escherichia coli strain BL21
recombinant enzyme
recombinant enzyme from Escherichia coli
-
recombinant FMN cofactor-bound His-tagged enzyme from Escherichia coli strain XL1-Blue by affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
A0A073KHN9
the GST-AzoR protein is purified by glutathione Sepharose 4B, the GST tag is removed by digestion with thrombin
-
via the hexa-histidine tag