1.7.1.6: azobenzene reductase

This is an abbreviated version!
For detailed information about azobenzene reductase, go to the full flat file.

Word Map on EC 1.7.1.6

Reaction

N,N-dimethyl-1,4-phenylenediamine
+
aniline
+ 2 NADP+ =
4-(dimethylamino)azobenzene
+ 2 NADPH + 2 H+

Synonyms

acpD, aerobic azoreductase, Azo 1, azo dye reductase, azo reductase, azo-dye reductase, AzoA, AzoB, AzoC, AzoEF1, AzoI, AzoII, AzoR, AzoR1, azoreductase, azoreductase 1, azoreductase B, azoreductase C, AZR, AzrA, AzrB, AzrC, AzrG, AzrS, bsAzoR, BTI1, class 1 azoreductase, class 2 azoreductase, dibromopropylaminophenylazobenzoic azoreductase, dimethylaminobenzene reductase, EC 1.6.6.7, ecAzoR, EF0404, efAzoR, EF_0404, flavin mononucleotide-containing azoreductase, flavin-dependent azoreductase, FMN-dependent NAD(P)H azoreductase, FMN-dependent NAD(P)H nitroreductase, FMN-dependent NADH-azo compound oxidoreductase, FMN-dependent NADH-azoreductase, methyl red azoreductase, N,N-dimethyl-4-phenylazoaniline azoreductase, NAD(P)H:1-(4'-sulfophenylazo)-2-naphthol oxidoreductase, NADH driven FMN dependent azoreductase, NADH-azoreductase, NADPH-dependent azoreductase Azr, NADPH-flavin azoreductase, NADPH2-dependent azoreductase, NC-reductase, new Coccine (NC)-reductase, nicotinamide adenine dinucleotide (phosphate) azoreductase, Orange I azoreductase, Orange II azoreductase, oxygen insensitive intracellular azoreductase, p-aminoazobenzene reductase, p-dimethylaminoazobenzene azoreductase, paAzoR1, PAMD_1, PpAzoR, reductase, azobenzene, rsAzoR, SO 4396

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.6 azobenzene reductase

Specific Activity

Specific Activity on EC 1.7.1.6 - azobenzene reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00191
-
activity in liver microsomes
0.0028
-
continuous assay for hepatic microsomal azo reductase
0.003
0.0035
-
activity in caecal suspension with amaranth; activity in liver microsomes with amaranth
0.004
-
activity in liver microsomes with carmoisine; activity in liver microsomes with Fast Red E
0.008
-
activity in liver microsomes with Ponceau 4R
0.011
-
activity in liver microsomes after flavin treatment
0.014
-
purification octyl-sepharose 4 fast flow
0.015
-
crude cell extract
0.045
-
activity in liver supernatant fraction
0.05
-
azoreductase II, with tartrazine
0.053
-
activity in liver supernatant fraction
0.054
-
activity in caecal suspension with carmoisine
0.06
-
activity in liver supernatant fraction
0.065
-
azoreductase II, with orange II
0.067
-
activity in liver supernatant fraction
0.07
-
azoreductase II, with amaranth
0.076
-
activity in caecal suspension with Fast Red E
0.087
-
activity in liver supernatant fraction
0.112
-
azoreductase I, with orange II
0.129
-
activity in caecal suspension with Ponceau 4R
0.14
-
after purification
0.19
-
azoreductase I, with amaranth
0.2
cosubstrate NADPH, pH 7.4, 30°C
0.212
-
azoreductase I, with tartrazine
0.224
-
purification DEAE Bio gel
0.315
-
azoreductase II, with ponceau SX
0.32
-
azoreductase I, with ponceau SX
0.626
-
purification step affinity gel
0.65
-
extract from host strain with Methyl red and NADH as substrates, pH 8.0, 40°C
1.1
-
purified recombinant enzyme with Red dye III and NADH as substrates, pH 8.0, 40°C
2.8
purified enzyme, pH 7.1 with 1-(4'-carboxyphenylazo)-4-naphthol and NADH as substrates
2.9
D5HN83
crude extract, using Orange I as the substrate with NADH as proton donor, pH 6.8, 45°C
3.55
-
pH 8.0, 45°C, crude enzyme, substrate reactive black 5
3.575
substrate direct Blue 15, cofactor NADPH
3.58
substrate direct blue 15, cosubstrate NADPH, pH 7.0, 25°C
3.699
substrate direct Blue 15, cofactor NADH
3.7
substrate direct blue 15, cosubstrate NADH, pH 7.0, 25°C
5.8
-
crude cell extract
7.6
-
recombinant enzyme at 50°C with roccelin
8.5
-
extract from Escherichia coli containing recombinant enzyme with Methyl red and NADH as substrates, pH 8.0, 40°C
10.1
D5HN83
purified enzyme, using Orange I as the substrate with NADH as proton donor, pH 6.8, 45°C
10.8
purified enzyme, pH 7.1 with 1-(4'-carboxyphenylazo)-2-naphthol and NADH as substrates
11.7
-
native enzyme at 70°C with roccelin
12.6
-
purified recombinant enzyme with Red dye II and NADH as substrates, pH 8.0, 40°C
15.4
-
cofactor FAD, pH not specified in the publication, temperature not specified in the publication
17
substrate Congo red, cosubstrate NADPH, pH 7.0, 25°C
17.02
substrate congo red, cofactor NADPH
18.18
substrate orange II, cofactor NADH
18.2
substrate orange II, cosubstrate NADH, pH 7.0, 25°C
19.8
-
purified native enzyme, pH 7.0, 30°C
20.3
-
purified recombinant enzyme with Red dye I and NADH as substrates, pH 8.0, 40°C
20.7
substrate Ponceau BS, cofactor NADH; substrate ponceau BS, cosubstrate NADH, pH 7.0, 25°C
23.2
substrate acid red 88, cosubstrate NADPH, pH 7.0, 25°C
23.23
substrate acid red 88, cofactor NADPH
24.29
substrate methyl red, cofactor NADPH
24.3
substrate methyl red, cosubstrate NADPH, pH 7.0, 25°C
25.8
cosubstrate NADH, pH 7.4, 30°C
26
-
pH not specified in the publication, temperature not specified in the publication
26.9
-
cofactor FMN, pH not specified in the publication, temperature not specified in the publication
38.2
C0STY0
substrate methyl red
38.6
C0STY0
substrate methyl red
63.9
-
purified enzyme, pH 7.1, 23°C, with Methyl red and NADH as substrates
76.6
substrate acid red 88, cosubstrate NADH, pH 7.0, 25°C
76.63
substrate acid red 88, cofactor NADH
78.9
-
purified recombinant enzyme with Methyl red and NADH as substrates, pH 8.0, 40°C
139.63
-
pH 8.0, 45°C, purified enzyme, substrate reactive black 5
153.16
purified wild-type AZR, 40°C, pH 7.0, substrate methyl red
156.1
pH 6.5, 25°C
160.62
purified recombinant fusion protein AZR-GDH, 40°C, pH 7.0, substrate methyl red
184.6
presence of 25 microM FMN, pH 6.5, 25°C
254
A0A073KHN9
purified recombinant enzyme, pH 6.5, 22°C, substrate congo red
505
-
purification step Cibacron blue agarose 3G
626
-
after 209fold purification, using methyl red and NADPH as substrates, in 50 mM potassium phosphate buffer pH 7.0 at 25°C
682
substrate Congo red, cosubstrate NADH, pH 7.0, 25°C
682.2
substrate congo red, cofactor NADH
5095
substrate methyl red, cofactor NADH; substrate methyl red, cosubstrate NADH, pH 7.0, 25°C
5152
A0A073KHN9
purified recombinant enzyme, pH 6.5, 22°C, substrate methl orange
17830
-
orange II azoreductase, strain KF46
97153
A0A073KHN9
purified recombinant enzyme, pH 6.5, 22°C, substrate methl red
additional information
C0STY0
substrate 1-(2-pyridylazo)-2-naphthol, relative activity 0.64%, methyl red 100%; substrate 1-(2-pyridylazo)-2-naphthol, relative activity 23.2%, methyl red 100%; substrate acid red 88, relative activity 0.12%, methyl red 100%; substrate acid red 88, relative activity 12.2%, methyl red 100%; substrate ethyl red, relative activity 113%, methyl red 100%; substrate ethyl red, relative activity 146%, methyl red 100%; substrate methyl orange, relative activity 0.21%, methyl red 100%; substrate methyl orange, relative activity 0.93%, methyl red 100%; substrate methyl red, relative activity 100%; substrate methyl red, relative activity 100%; substrate new cossin, relative activity 0.1%, methyl red 100%; substrate orange II, relative activity 0.01%, methyl red 100%; substrate orange II, relative activity 0.29%, methyl red 100%; substrate orange I, relative activity 0.03%, methyl red 100%; substrate orange I, relative activity 1.26%, methyl red 100%; substrate sunset yellow FCF, relative activity 0.16%, methyl red 100%