nitrous-oxide reductase

This is an abbreviated version!
For detailed information about nitrous-oxide reductase, go to the full flat file.

Word Map on EC


+ 2 ferricytochrome c =
Nitrous oxide
+ 2 ferrocytochrome c + 2 H+


HdN2OR, McoP, MhN2OR, multicopper oxidase, N(2)OR, N2O reductase, N2OR, nitrous oxide reductase, NosZ, PpN2OR, PsN2OR, Sden_2219, SdN2OR, Z-type N2OR


     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
       nitrous-oxide reductase


Purification on EC - nitrous-oxide reductase

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a pink form is isolated when the purification is done aerobically, a purple form when the purification is under anaerobic conditions
ammonium sulfate, DEAE-Sephadex A-50, Sephacryl S-200
anaerobical purification of N2OR under an Ar atmosphere by three different steps of anion exchange chromatography, followed by cytochrome c affinity chromatography and ultrafiltration
DEAE cellulose column chromatography, hydroxyapatite column chromatography, Mono Q10 anion-exchange column chromatography, and Sephadex S-200 gel filtration
DEAE-FF ion exchange chromatography and Resource Q column chromatography
HiTrap Q Sepharose anion exchange chromatography
isolation of the enzyme in the purple form, in which the copper centre is in the oxidized [2Cu2+:2Cu+] redox state and is redox active. Isolation of this form in the presence of oxygen from a microaerobic culture in the presence of nitrate and also from a strictly anaerobic culture
native enzyme is a three-step procedure
of anaerobically grown cells
partial purification under anaerobic conditions
purification aerobically at 4°C
purification under anaerobic conditions
recombinant His6-tagged enzyme and Strep-tagged enzyme from Escherichia coli strain BL21(DE3) with or without bound calcium by metal affinity and streptavidin chromatography, respectively, followed by gel filtration
recombinant metal-free apo protein
two forms a purple and a blue form