1.7.2.4: nitrous-oxide reductase

This is an abbreviated version!
For detailed information about nitrous-oxide reductase, go to the full flat file.

Word Map on EC 1.7.2.4

Reaction

nitrogen
+
H2O
+ 2 ferricytochrome c =
Nitrous oxide
+ 2 ferrocytochrome c + 2 H+

Synonyms

HdN2OR, McoP, MhN2OR, multicopper oxidase, N(2)OR, N2O reductase, N2OR, nitrous oxide reductase, NosZ, PpN2OR, PsN2OR, Sden_2219, SdN2OR, Z-type N2OR

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.4 nitrous-oxide reductase

Specific Activity

Specific Activity on EC 1.7.2.4 - nitrous-oxide reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.4
-
fully oxidized enzyme isolated from aerobic growth conditions, pH 7.6, temperature not specified in the publication
1
-
ascorbate-reduced phenanzine methosulphate electrode assay
1.6
-
anaerobic form pre-reduced with ascorbate
1.97
-
wild type enzyme
122
-
anaerobic form
124
-
purified enzyme, pH 8.0, 25C
2.8
-
anaerobic form pre-reduced with dithionite
23
-
blue-coloured form
27
-
pH 7.1, 25C
29
-
HdN2OR form A, i.e. reduced form
3.3
-
photoreduced methyl viologen spectrophotometric assay
3.52
-
single mutant nirX
3.63
-
double mutant nosXnirX
3.7
-
dithionite-reduced methyl viologen electrode assay
45
-
HdN2OR form A, i.e. oxidized form
453
enzyme purified from transgenic Nicotiana tabacum, pH 7.1, 37C
5.5
-
wild-type
55
-
purple coloured form
61.7
-
after preincubation in 2 mM reduced benzyl viologen
78
-
anaerobic form
8.5
-
aerobic form pre-oxidized with ferricyanide
8.8
-
aerobic form pre-reduced with dithionite
88
-
fully reduced enzyme isolated from aerobic growth conditions, pH 7.6, temperature not specified in the publication
92.4
-
fully reduced enzyme isolated from aerobic growth conditions, pH 7.6, temperature not specified in the publication
additional information
-
Vmax N2O 0.2 - 0.5 micromol/min/mg, pH 7.1, 25C