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1.7.2.5: nitric oxide reductase (cytochrome c)

This is an abbreviated version!
For detailed information about nitric oxide reductase (cytochrome c), go to the full flat file.

Word Map on EC 1.7.2.5

Reaction

nitrous oxide
+
2 ferricytochrome c
 +
H2O
= 2 nitric oxide + 2 ferrocytochrome c + 2 H+

Synonyms

Anor, ba3-oxidase, c-type nitric oxide reductase, cNOR, Cnor1, Cnor2, cytochrome c dependent nitric oxide reductase, cytochrome c dependent NOR, cytochrome c type NOR, cytochrome c-dependent nitric oxide reductase, cytochrome c-dependent NO reductase, EC 1.7.99.7, flavorubredoxin, FlRd, Fnor, FprA, membrane-integrated nitric oxide reductase, nitric oxide reductase, nitric oxide reductase cytochrome, nitric oxide-reductase, nitrogen oxide reductase, NO reductase, NO-reductase, NOR, NorB, NorBC, NorC, NorCB, NorZ, P450nor, qCuANOR, respiratory nitric oxide reductase, respiratory NO reductase, S-NOR, scavenging nitric oxide reductase, Tnor

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.5 nitric oxide reductase (cytochrome c)

Purification

Purification on EC 1.7.2.5 - nitric oxide reductase (cytochrome c)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate, Resource Q, Resource PHE, Superdex 200
DEAE column chromatography and hydroxyapatite column chromatography
Marinobacter nauticus
-
DEAE-Sepharose CL-6B anion-exchange chromatography, Sephacryl S-300 gel filtration, and Mono Q anion-exchange chromatography. Enzyme is solubilized with sucrose monodecanoate from the membranes of Roseobacter denitrificans grown aerobically under light conditions, and purified to electrophoretic homogeneity
-
Q-Sepharose anion exchange chromatography
-
Q-Sepharose column chromatography and HiTrap chelating Sepharose column chromatography
-
recombinant enzyme from Escherichia coli by solubilization with 1% w/v n-dodecyl-beta-D-maltoside, ultracentrifugation, and anion exchange chromatography
recombinant FlRd
-
recombinant His-tagged NorH, wild-type cNOR, and mutant cNOR lacking norH by nickel affinity chromatography from Escherichia coli
D5GU62; D5GU63
recombinant wild-type and mutant NorC subunits from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and again ion exchange chromatography
T7 tag affinity chromatography and Superdex75 gel filtration
-
using detergent lauryl maltoside
-
water-soluble NorC domain expressed in Escherichia coli
-
with n-dodecyl-beta-D-maltoside
-