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1.8.1.2: assimilatory sulfite reductase (NADPH)

This is an abbreviated version!
For detailed information about assimilatory sulfite reductase (NADPH), go to the full flat file.

Word Map on EC 1.8.1.2

Reaction

hydrogen sulfide
+ 3 NADP+ + 3 H2O =
sulfite
 + 3 NADPH + 3 H+

Synonyms

CNG03990, coenzyme F420-dependent sulfite reductase, CysI, CysIJ, desulforubidin, EC 1.8.99.1, Fsr, H2S-NADP oxidoreductase, MET10, NADPH-dependent assimilatory sulfite reductase, NADPH-dependent sulfite reductase, NADPH-sulfite reductase, reductase, sulfite (reduced nicotinamide adenine dinucleotide phosphate), SIR, SIR-FP, SIR-HP, SiR/SiRHP, SIRHP, sulfite reductase, sulfite reductase hemo-subunit

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.2 assimilatory sulfite reductase (NADPH)

Cloned

Cloned on EC 1.8.1.2 - assimilatory sulfite reductase (NADPH)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
alpha and beta subunits
-
expressed in LMG194 Escherichia coli cells
-
FMN-binding domain of flavoprotein component
-
gene MET10, DNA and amino acid sequence determination and analysis
homology to cysI of Escherichia coli, i.e. alpha subunit
-
NADPH- and FAD-binding domain of flavoprotein component
-
the MET5 and Met10 coding sequences are cloned into the vector pGEM-T-easy and subsequently into the centromeric Saccharomyces cerevisiae expression plasmid pRS416
-
the sulfite reductase hemo-subunit is cloned into the vector pLM1 for expression in Escherichia coli BL21DE3 cells
-