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analysis
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development of a blue native-PAGE-based method for isolation of enzymatically active DLDH from animal tissues and visualization as well as quantification of its diaphorase activity using the NADH/nitroblue tetrazolium detection system
drug development
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a homology model for PfaE3 reveals an extra anti-parallel beta-strand at the position where human E3BP (E3-binding protein) interacts with E3, a parasite-specific feature that may be exploitable for drug discovery against pyruvate dehydrogenase complex, PDC. Plasmodium PDC is essential for parasite survival in the mosquito vector and for late liver stage development in the human host, suggesting its suitability as a target for intervention strategies against malaria
degradation
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conservation of the Cys-45 residue in human E3 is essential to the efficient catalytic function of the enzyme
degradation
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decreased activity of DLDH induced by valproic acid metabolites may, at least in part, account for the impaired rate of oxygen consumption and ATP synthesis in mitochondria if 2-oxoglutarate or glutamate are used as respiratory substrates, thus limiting the flux of these substrates through the citric acid cycle
degradation
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DLD is required for hamster acrosome reaction
degradation
N286 and D320 play a role in the catalytic function of the E3
degradation
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S456 and E431 form a catalytic dyad in the DLD monomer, whereas H450, by forming a hydrogen bond with E431, may decrease the ability of E431 to polarize the hydroxyl group of S456
degradation
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shows flavin reductase activity with moderate diaphorase activity
degradation
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shows NADH-dependent tellurite reductase activity in vitro
degradation
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shows NADH-dependent tellurite reductase activity in vitro
degradation
shows NADH-dependent tellurite reductase activity in vitro
degradation
shows NADH-dependent tellurite reductase activity in vitro
degradation
shows NADH-dependent tellurite reductase activity in vitro
degradation
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shows strong diaphorase activity
degradation
T148 is not important to E3 catalytic function, whereas R281 plays a role in the catalytic function of E3
diagnostics
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Bacillus sphaericus DLD-diaphorase exhibits considerable potential to be used as a component of diagnostic tests for the quantification of metabolites
diagnostics
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Bacillus sphaericus DLD-diaphorase exhibits considerable potential to be used as a component of diagnostic tests for the quantification of metabolites
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medicine
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mutations of the enzyme cause the often-fatal human disease known as E3 deficiency
medicine
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brain DLDH expression and activity undergo independent postnatal maturational increases. Senescence does not confer any detectable change in the activity of DLDH or its susceptibility to inactivation by mitochondrial oxidative stress
medicine
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dihydrolipoyl dehydrogenase is an important source of reactive oxygen species leading to life span limitation
medicine
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direct involvement of LpdC in the prolonged retention of coronin-1 on the phagosome, thus promoting phagosome maturation arrest. Under the influence of IFNgamma, LRG-47 is recruited to the phagosome and mediates dislocation of coronin-1 leading ultimately to phagosome acidification and recruitment of lysosomal vacuoles and phagolyosome fusion
medicine
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direct involvement of LpdC in the prolonged retention of coronin-1 on the phagosome, thus promoting phagosome maturation arrest. Under the influence of IFNgamma, LRG-47 is recruited to the phagosome and mediates dislocation of coronin-1 leading ultimately to phagosome acidification and recruitment of lysosomal vacuoles and phagolyosome fusion
medicine
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linkage and association analysis of diplotypes in the DLD gene with Alzheimer's from the National Institute of Mental Health-National Cell Repository for Alzheimer's disease and Italian data series, controlling for Gender and ApoE e4 status. Significant evidence of association of DLD with Alzheimer's disease. Combining the linkage and association study results for DLD together, leads to a p-value that is more significant than any of the individual p-value results for DLD. Only with sufficiently large sample sizes it is possible to rule out whether the DLD gene is in fact associated with Alzheimers disease
medicine
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lipoamide dehydrogenase activity and metallothionein levels may be critical for dopaminergic neuronal survival in Parkinson's disease. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine can affect the lipoamide dehydrogenase activity and metallothionein content to exert its neurotoxicity
medicine
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NADH diaphorase staining can establish tissue non-viability after radiofrequency ablation, but the timing of staining after treatment must be considered when interpreting results to avoid false positive tests. Tissue that is apparently viable by NADH diaphorase staining within 2.5 hours of radiofrequency ablation may in fact have been ablated
medicine
enzyme is surface-exposed and contributes to survival of Pseudomonas aeruginosa in human serum. Enzyme binds the four human plasma proteins, Factor H, factor H-like protein-1, complement factor H-related protein 1, and plasminogen. Factor H contacts the enzyme via short consensus repeats 7 and 18-20. Factor H, factor H-like protein-1, and plasminogen when bound to enzyme are functionally active. Bacterial survival is reduced when the enzyme is blocked on the surface prior to challenge with human serum. Similarly, bacterial survival is reduced up to 84% when the bacteria are challenged with complement active serum depleted of factor H, factor H-like protein-1, and complement factor H-related protein 1
medicine
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due to its TiO2 binding ability, the enzyme may serve as a molecular bridge between Ti-based medical structures and human tissues
medicine
due to its TiO2 binding ability, the enzyme may serve as a molecular bridge between Ti-based medical structures and human tissues
medicine
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Arg-Gly-Asp-modified enzyme enhances the adhesion of bone forming cells to titanium dioxide implant surfaces
medicine
the enzyme is a DNA chelating agent. Enzyme binding to DNA presents a moonlight activity which may be used for DNA alkylating in cancer treatment
medicine
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lipoamide dehydrogenase activity and metallothionein levels may be critical for dopaminergic neuronal survival in Parkinson's disease. 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine can affect the lipoamide dehydrogenase activity and metallothionein content to exert its neurotoxicity
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medicine
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enzyme is surface-exposed and contributes to survival of Pseudomonas aeruginosa in human serum. Enzyme binds the four human plasma proteins, Factor H, factor H-like protein-1, complement factor H-related protein 1, and plasminogen. Factor H contacts the enzyme via short consensus repeats 7 and 18-20. Factor H, factor H-like protein-1, and plasminogen when bound to enzyme are functionally active. Bacterial survival is reduced when the enzyme is blocked on the surface prior to challenge with human serum. Similarly, bacterial survival is reduced up to 84% when the bacteria are challenged with complement active serum depleted of factor H, factor H-like protein-1, and complement factor H-related protein 1
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medicine
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due to its TiO2 binding ability, the enzyme may serve as a molecular bridge between Ti-based medical structures and human tissues
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additional information
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a mutation in LPD leads to a pyruvate dehydrogenase complex that is less sensitive to inhibition by NADH, allowing the enzyme to function in an anaerobic culture, which changes the fermentation profile of the mutant. Presence and functional activity of such an NADH-insensitive pyruvate dehydrogenase may have significant unexplored physiological and biotechnological applications
additional information
high stability of rBfmBC may make it useful for practical use
additional information
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high stability of rBfmBC may make it useful for practical use
additional information
metabolic context(s) of DLDHs remains an open question
additional information
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metabolic context(s) of DLDHs remains an open question
additional information
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the lipoyl protein domain (but not lipoic acid alone) plays a regulatory role in the enzymatic characteristics of pneumococcal DLDH