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1.8.1.7: glutathione-disulfide reductase

This is an abbreviated version!
For detailed information about glutathione-disulfide reductase, go to the full flat file.

Word Map on EC 1.8.1.7

Reaction

2 glutathione +

NADP+
=
glutathione disulfide
 +
NADPH
 +
H+

Synonyms

At3g54660, EC 1.6.4.2, GLR, glutahione reductase, glutathione disulfide reductase, glutathione reductase, glutathione reductase (NADPH), glutathione reductase 3, glutathione reductase Glr1, glutathione S-reductase, glutathione: NADP(+) oxidoreductase, glutathione: NADP+ oxidoreductase, glutathione:NADP+ oxidoreductase, Gor, GOR1, GOR2, GR, GR1, GR2, Gr3, GRase, GRase-1, GRd, GSH reductase, GSHR, Gsr, GSR-1, GSSG reductase, HCOI_01258400, hGR, HvGR1, HvGR2, multifunctional thioredoxin-glutathione reductase, NADPH-glutathione reductase, NADPH-GSSG reductase, NADPH-reduced GR, NADPH: oxidized glutathione oxidoreductase, NADPH:oxidized glutathione oxidoreductase, NADPH:oxidized-glutathione oxidoreductase, OBP29, PfGR, psgr, PtGR1.1, PtGR1.2, PtGR2, reductase, glutathione, SpGR, TaGR1, TaGR2, TGR, thioredoxin glutathione reductase, thioredoxin/glutathione reductase, TrxR3

ECTree

     1 Oxidoreductases
         1.8 Acting on a sulfur group of donors
             1.8.1 With NAD+ or NADP+ as acceptor
                1.8.1.7 glutathione-disulfide reductase

Storage Stability

Storage Stability on EC 1.8.1.7 - glutathione-disulfide reductase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, in the dark, 10% glycerol, 5 mM NADP+, for at least 1 year
-
-20°C, 1 year
-
-20°C, 2 years
-
-20°C, 2-3 months
Achromobacter starkeyi
-
-20°C, 20 mM potassium phosphate, pH 7.5, 1 mM EDTA, 10% glycerol, months
-
-20°C, pH 6.8, 0.05 M potassium phosphate buffer, indefinitely stable
-
-20°C, several weeks
-
-20°C, with several freeze-thawing cycles, more than 2 years, stable
-
-28°C, 50 mM phosphate buffer, a few months
-
-70°C, pH 7.0, 1 mM EDTA, 1 mM dithiothreitol, 0.020 mM FAD, 5 mM NADP+
-
0-4°C, 1 month, no appreciable effect of freezing and thawing on enzyme activity
-
0-4°C, crystals in buffer, several months
-
0°C, 6 days
-
25°C, in the dark
-
4°C, 50 mM potassium phosphate, pH 7.4, 50% loss of activity after 10 days
-
4°C, activation after storage over days, months or years
4°C, concentrated purified enzyme, 0.05% NaN3, 50 mM potassium phosphate, pH 7.4, 2 mM EDTA, 150 mM NaCl, 2 mM glutathione, quite stable
-
4°C, pH 7.5, 50 mM sodium phosphate buffer, 1 mM 2-mercaptoethanol, 1 mM EDTA
-
4°C, pure enzyme without stabilizers such as glycerol, 8 days, 25% loss of activity
-
4°C, several weeks
-
concentrated solution, years
in refrigerator, several months
-